Expert Details

Expert in Allergenicity Assessment of Genetically Modified Foods

Expert ID: 725131 California, USA

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Protein isolated from different sources and introduced in plants for abiotic stress tolerance and antifungal activity was cloned and expressed in pET 22b+ vector and purified by chromatographic (Ni-NTA affinity chromatograpy) technique. Safety of proteins was assessed by in-vivo and in-vitro studies for allergenicity and toxicity. Safety of transgenic plant having siRNA for protection against leaf curl virus disease was done for assessment allergenicity and toxicity by in-vitro assays and animal model studies.

Extensive knowledge of protein extraction, purifications and standardization of protocols. Comparative proteomics analysis by Two dimensional analysis electrophoresis has been done to analyze the GM and native plants proteins profile at the level of proteins and by IgE Immunoblot with mustard positive patient’s sera.
In-vitro digestibility and heat stability of proteins was also assessed.


Safety of foods developed by biotechnology has been studied. Animal model was developed by systemic exposure of GM and native plant protein in Balb/c mice to study the immunological responses. Tissue histolopathological analysis was done to study the intracellular inflammation. Spleen cells isolation for Th1/Th2 cytokines analyses was performed.
Allergenicity and protein toxicity of purified proteins was also assessed using in-vitro methods and animal model studies.

He consulted the labs for standardizing animal model studies for immune response analysis. He also consulted for mutational analyses for making hypoallergens.He consulted labs of plant biotech for potential genes in transgenic crops and also the ways of do their initial assessment and also the safety assessment of the modified transgenic crops expressing a foreign gene.

Education

Year Degree Subject Institution
Year: 2008 Degree: Ph.D Subject: Biotechnology Institution: IGIB(CSIR)
Year: 2001 Degree: M.Sc. Subject: Microbiology Institution: Gurukul University, India
Year: 1999 Degree: B.Sc. Subject: Chemistry, Botany, Zoology Institution: Gurukul University, India
Year: 2004 Degree: PG Diploma in Patents Law Subject: IPR and Patenting Institution: NALSAR, India

Work History

Years Employer Title Department Responsibilities
Years: 2010 to 2012 Employer: Stanford University Title: Postdoc Fellow Department: Center for Narcolepsy Responsibilities: He is responsible for analyzing the role of influenza virus in initiating novel diseases.
Years: 2009 to 2010 Employer: University of Southern California Title: Postdoctrate Research Associate Department: MMI Division Responsibilities: He is responsible for analyzing Asthma pathogenesis in viral infection specially influenza A using invivo and invitro methods.
Years: 2006 to 2008 Employer: IGIB(CSIR) Title: Senior Research Fellow Department: Allergy and Immunology Division Responsibilities: He is responsible for developing novel protocols for allergenicity assessment of GM foods. He has done mutational analysis for development of hypoallergenic mutants for allergens.
Years: 2002 to 2006 Employer: IGIB(CSIR) Title: Project Fellow Department: Allergya and Immunology Responsibilities: He is responsible for the safety assessment of different proteins expressed in GM foods. Proteomics analysis and animal model studies for comparative safety assessment of native and GM plants.

Government Experience

Years Agency Role Description
Years: 2002 to 2008 Agency: IGIB(CSIR) Role: Research Description: He is responsible of developing novel protocols for allergenicity and toxicity assessment of GM foods.

International Experience

Years Country / Region Summary
Years: 2009 to 2010 Country / Region: USA Summary: He is working on developing novel methods for allergic asthma analysis. He is particularly helping in designing protocols for developing asthma models in mice .
Years: 2010 to 2012 Country / Region: USA/ CA Summary: He is working towards analyzing the role of influenza virus in initiating novel diseases.

Career Accomplishments

Associations / Societies
Life member: Indian College of Allergy and Applied Immunology (ICAAI);
Junior member: European Academy of Allergology and Clinical Immunology (EAACI);
Student member: Society of Toxicology (SOT).
Licenses / Certifications
Passed DOEACC “O” LEVEL Examination in Jan 1999. Familiar with Dos, Win, FoxPro, etc.
Patenting And IPR Knowledge intellectual property rights from World Intellectual property Right organization (WIPO).
Professional Appointments
Jan 2009 till date: Postdoc fellow, USC, LA, USA: June 2006 - June 2008: Senior Research Fellow, Allergy and Immunology lab, IGIB (CSIR);
July 2003 – May 2006: Project Fellow, Allergy and Immunology lab, IGIB (CSIR);
June 2002 – April 2003: Project Fellow, Microbiology division, IGIB (CSIR).
Awards / Recognition
APAAACI Travel Grant Award
to attend the conjoint meeting of the 7th Asia Pacific Congress of Allergy, Asthma and Clinical Immunology and the 20th World Allergy Congress held in December 2007 in Bangkok, Thailand.
Medical / Professional
Senior Research Fellowship
Indian Council of Medical Research, in June 2006.

Publications and Patents Summary
He has 8 research publications in peer review journals, 2 Review articles, 2 book chapters, 3 international and 2 national abstracts.

Additional Experience

Training / Seminars
He has given oral presentation of his work at World Allergy Congress-2007 in Bangkok.
He has delivered lectures on GM food safety at various Indian conferences.
Marketing Experience
He has done the WIPO certification in general patenting and IPR. He has developed manuals for allergenicity assessment of GM foods in animal models, Proteomics analysis etc.
Other Relevant Experience
Animal model studies, proteomics, Molecular Biology,
IPR and Patenting in biotechnology

Language Skills

Language Proficiency
English Excellent in English

Fields of Expertise

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