Expert Details

Expert in Biomolecular Research Resource

Expert ID: 717764 California, USA

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AMINO ACIDS; PEPTIDES; PROTEINS; PROTEIN PURIFICATION. Expert has devoted a considerable portion of his career to providing the capability to purify, sequence, and synthesize proteins. He has helped develop the technologies and protocols needed as well as implement the ideas provided by others. While samples and sequences from many investigators and sources have been successfully completed, Expert has emphasized techniques to sequence rare, difficult to purify, and otherwise difficult to sequence proteins and peptides.

PROTEIN STRUCTURE. Protein sequencing has been the principal focus of a section of Expert's laboratory. He has led the development of technologies, protocols, and strategies needed to determine the primary structure of rare, difficult to purify proteins and peptides. High sensitivity operation and long runs are standard procedures f.

PEPTIDE SYNTHESIS. Expert's lab has furnished custom synthetic peptides to many research labs and research and development facilities. He is knowledgeable of the characterization, purification, and refolding necessary for successful preparation of peptides with biological activity. His laboratory offers support to help plan the use of synthetic peptides and to select the sequence for custom syntheses and peptide use.

NUCLEIC ACIDS; RNA; DNA SEQUENCING; DNA SYNTHESIS; GENETIC ENGINEERING. Expert has directed the laboratory that has provided nucleic acid synthesis support for many applications in molecular biology, genetic engineering, biochemistry, and other fields for more than ten years. The facility has synthesized more than 10,000 oligomers and provides DNA sequencing using automated instruments to many researchers and research and development labs. Protocols are available to handle templates from diverse sources, in different vectors, single or double stranded. Mapping based on fluorescent probes to small repeats or unique sequences is performed on an automated DNA analyzer. Template purification is the primary limitation to sequencing and mapping. Maintaining working protocols is a primary focus of Expert's laboratory.

AMINO ACID SEQUENCING. Expert has sequenced proteins of diverse types using several implementations of Edman degradation for scientists around the world. Proteins or peptides purified by many different laboratories using a variety of strategies have been successfully sequenced form the amino terminus or from an internal fragmentation site.


Through outsourcing, I have managed research programs where only part time staff were available in house.

Expert may consult nationally and internationally, and is also local to the following cities: Los Angeles, California - San Diego, California - Long Beach, California - Santa Ana, California - Anaheim, California - Riverside, California - Glendale, California - Huntington Beach, California - San Bernardino, California - Chula Vista, California

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Education

Year Degree Subject Institution
Year: 1971 Degree: Post-doctoral Subject: Physiological Chemisty Institution: University of Wisconsin - Madison
Year: 1968 Degree: PhD Subject: Genetics Institution: University of Wisconsin - Madison
Year: 1963 Degree: MS Subject: Genetics Institution: University of Wisconsin - Madison
Year: 1962 Degree: BS Subject: Biology Institution: Blackburn College

Work History

Years Employer Title Department Responsibilities
Years: 1998 to Present Employer: Undisclosed Title: Consultant Department: Responsibilities: Design research programs for small and startup biopharmaceutical companies, recruit CROs, negotiate and manage contracts, integrate resulting materials and data into the in house program.
Years: 1995 to 1998 Employer: University Of California, Irvine Title: Director, Biotechnology Resource Facility Department: Responsibilities: Develop state-of-the-art capabilities in protein and DNA analysis in support of campus research programs.
Years: 1985 to 1995 Employer: University of Wisconsin Biotechnology Center Title: Director-Protein/DNA Sequence/synthesis Facility, Department: Responsibilities: Provide suite of research resources to provide world class capabilities in protein synthesis, purification and analysis and DNA synthesis and analysis, molecular biology, hybridoma and transgenic technologies.
Years: 1971 to 1985 Employer: McArdle Laboratory for Cancer Research Title: Program Coordinator, Department: Responsibilities: Develop and deliver high performance protein and DNA sequencing and peptide and DNA synthesis.
Years: 1971 to 1985 Employer: Department of Physiological Chemistry Title: Project Associate, Department: Responsibilities: Develop protein sequencing capabilities in support of molecular evolution studies.

Career Accomplishments

Associations / Societies
AAAS,AAUP,ABRF,ACS,ALMA,ASBMB,ASMS,GSA
Professional Appointments
Executive Board, ABRF; Associate Editor, Journal of Biomolecular Techniques; Visiting Professor, Universidade de Brasília, Brazil; Visiting Professor, Facultad de Farmacia y Bioquímica, Buenos Aires, Argentina
Publications and Patents Summary
Expert has 40 publications on research topics and delivery of research resources.

Fields of Expertise

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