Expert in Biomolecular Research Resource
Expert ID: 717764 California, USA
PROTEIN STRUCTURE. Protein sequencing has been the principal focus of a section of Expert's laboratory. He has led the development of technologies, protocols, and strategies needed to determine the primary structure of rare, difficult to purify proteins and peptides. High sensitivity operation and long runs are standard procedures f.
PEPTIDE SYNTHESIS. Expert's lab has furnished custom synthetic peptides to many research labs and research and development facilities. He is knowledgeable of the characterization, purification, and refolding necessary for successful preparation of peptides with biological activity. His laboratory offers support to help plan the use of synthetic peptides and to select the sequence for custom syntheses and peptide use.
NUCLEIC ACIDS; RNA; DNA SEQUENCING; DNA SYNTHESIS; GENETIC ENGINEERING. Expert has directed the laboratory that has provided nucleic acid synthesis support for many applications in molecular biology, genetic engineering, biochemistry, and other fields for more than ten years. The facility has synthesized more than 10,000 oligomers and provides DNA sequencing using automated instruments to many researchers and research and development labs. Protocols are available to handle templates from diverse sources, in different vectors, single or double stranded. Mapping based on fluorescent probes to small repeats or unique sequences is performed on an automated DNA analyzer. Template purification is the primary limitation to sequencing and mapping. Maintaining working protocols is a primary focus of Expert's laboratory.
AMINO ACID SEQUENCING. Expert has sequenced proteins of diverse types using several implementations of Edman degradation for scientists around the world. Proteins or peptides purified by many different laboratories using a variety of strategies have been successfully sequenced form the amino terminus or from an internal fragmentation site.
Through outsourcing, I have managed research programs where only part time staff were available in house.
|Year: 1971||Degree: Post-doctoral||Subject: Physiological Chemisty||Institution: University of Wisconsin - Madison|
|Year: 1968||Degree: PhD||Subject: Genetics||Institution: University of Wisconsin - Madison|
|Year: 1963||Degree: MS||Subject: Genetics||Institution: University of Wisconsin - Madison|
|Year: 1962||Degree: BS||Subject: Biology||Institution: Blackburn College|
|Years: 1998 to Present||Employer: Undisclosed||Title: Consultant||Department:|
Responsibilities:Design research programs for small and startup biopharmaceutical companies, recruit CROs, negotiate and manage contracts, integrate resulting materials and data into the in house program.
|Years: 1995 to 1998||Employer: University Of California, Irvine||Title: Director, Biotechnology Resource Facility||Department:|
Responsibilities:Develop state-of-the-art capabilities in protein and DNA analysis in support of campus research programs.
|Years: 1985 to 1995||Employer: University of Wisconsin Biotechnology Center||Title: Director-Protein/DNA Sequence/synthesis Facility,||Department:|
Responsibilities:Provide suite of research resources to provide world class capabilities in protein synthesis, purification and analysis and DNA synthesis and analysis, molecular biology, hybridoma and transgenic technologies.
|Years: 1971 to 1985||Employer: McArdle Laboratory for Cancer Research||Title: Program Coordinator,||Department:|
Responsibilities:Develop and deliver high performance protein and DNA sequencing and peptide and DNA synthesis.
|Years: 1971 to 1985||Employer: Department of Physiological Chemistry||Title: Project Associate,||Department:|
Responsibilities:Develop protein sequencing capabilities in support of molecular evolution studies.
|Associations / Societies|
|Executive Board, ABRF; Associate Editor, Journal of Biomolecular Techniques; Visiting Professor, Universidade de Brasília, Brazil; Visiting Professor, Facultad de Farmacia y Bioquímica, Buenos Aires, Argentina|
|Publications and Patents Summary|
|Expert has 40 publications on research topics and delivery of research resources.|
Fields of Expertise
amino acid, DNA sequencing, DNA synthesis, genetic engineering, peptide purification, peptide synthesis, protein, protein purification, protein sequencing, protein structure, protein synthesis, green fluorescent protein, neurotoxin exposure, amino acid sequence, amino acid analysis, synthetic DNA, facility evaluation, oligomer, high-performance liquid chromatography, DNA structure, peptide, site directed mutagenesis, oncology, liquid chromatography, hydrolysis, genetics, gel electrophoresis, electrophoresis, cloning technique, biotechnology