Expert Details

Expert in Bionanosciences, Nanomaterial interfacing & targeting, Cell biology & biophysics, Molecular imaging, Microscopy

Expert ID: 726604 California, USA

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The expert has been actively designing and developing surface chemistries and coatings that allow a controlled organic/inorganic interfacing of colloidal nanomaterials with biomolecules. Over the past 14 years, projects carried out by the expert have focused on engineering the surface of 2-20 nm nanostructures to specifically target molecules and structures of interest in living cells and animal models. Using multidisciplinary approaches, the expert has carried research projects all the way from the nanomaterial synthesis and development phase to actual biological applications. His work encompasses the wet-chemical synthesis of colloidal nanostructures, the solubilization, stabilization, and biofunctionalization of inorganic nanomaterials using small organic molecules, thin silica shell encapsulation or custom-designed peptides, the development of specific and high affinity bioconjugation strategies, the physical and biochemical characterization of hybrids nanomaterials by spectroscopy, TEM, AFM, FTIR, chromatography or electrophoresis, and the use of these nanomaterials for biological targeting and imaging in living cells and live animals.

In addition to his expertise in nanomaterials interfacing with biomolecules the expert has extensive experience in single molecule detection, ultra-high resolution fluorescence imaging including super-resolution imaging and single particle tracking in living cells and biological tissues. In particular, the expert continuously develops new fluorescent probes and imaging strategies to study biomolecules in their native environment and map cellular structures with nanometer resolution. The expert has firsthand experience in fluorescence microscopy including confocal, stage scanning, TIRF, wide field, and PALM/STORM microsopy, optics, fluorescent probes photophysics, protein engineering, protein expression and purification, protein and DNA bioconjugation, genetic engineering and molecular biology, cell culture, biochemistry of cell membranes, intracellular targeting and delivery techniques, fluorescence quantification diffusion analysis and image treatment.

The expert multidisciplinary research has attracted financial support by the National Institut of Health (NIH), the National Science Foundation (NSF), the Marie-Curie Actions and the European Molecular Biology organization (EMBO).

Expert may consult nationally and internationally, and is also local to the following cities: Los Angeles, California - Long Beach, California - Santa Ana, California - Anaheim, California - Riverside, California - Bakersfield, California - Glendale, California - Huntington Beach, California - San Bernardino, California - Oxnard, California

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Education

Year Degree Subject Institution
Year: 2007 Degree: PhD Subject: Biochemistry and Molecular Biology Institution: University of California Los Angeles
Year: 1999 Degree: BSc Subject: Biomedical Sciences Institution: Nottingham Trent University
Year: 1996 Degree: Diplome Universitaire de Technologie Subject: Genie Biologique, Analyses Biologiques et Biochimiques Institution: Institut Universitaire de Technologie Clermont-Ferrand

Work History

Years Employer Title Department Responsibilities
Years: 2019 to Present Employer: Undisclosed Title: Associate Professor Department: Biological Sciences, Chemistry and Physics and Astronomy Responsibilities: Principal Investigator of the Single Molecule Biophotonics Group
Years: 2011 to 2019 Employer: University of Southern California Title: Assistant Professor Department: Biological Sciences, Chemistry and Physics and Astronomy Responsibilities: Principal Investigator of the Single Molecule Biophotonics Group
Years: 2007 to 2011 Employer: Ecole Normale Superieure Title: Marie-Curie and EMBO Post-doctoral Fellow Department: Departement de Physique, Groupe Optique et Biologie Responsibilities: Expert develops fluorescent probes (dyes, fluorescent proteins, quantum dots…) and microscopy techniques for single molecule imaging and tracking in living cells and organisms.
Years: 2002 to 2007 Employer: University of California, Los Angeles Title: Research Assistant Department: Department of Chemistry and Biochemistry Responsibilities: Expert develops surface and colloidal chemistry's of semiconductor quantum dots for fluorescence imaging of biological samples. He designs and build optical set up for high sensitivity fluorescent microscopy and engineers proteins and high affinity pairs for single molecule microscopy in living cells. Expert also develops cell models for single molecule imaging and tracking with quantum dots and studies the diffusional dynamics of membrane proteins associated with micro-domains in living cells.
Years: 2001 to 2002 Employer: University of California, Berkeley Title: Research Assistant Department: Biophysics Responsibilities: Expert develops surface chemistry of nanomaterials and analytical techniques and processes for colloidal characterization of bio-compatible quantum dots. Expert also characterize quantum dot by single molecule fluorescence microscopy.
Years: 1999 to 2001 Employer: Lawrence Berkeley National Laboratory Title: Research assistant Department: Material Sciences and Physical Biosciences Division Responsibilities: Expert synthesizes semiconductor quantum dot probes and characterizes their photo-physical properties. He also develops bio-compatible surface chemistry and bio-labeling strategies.
Years: 1997 to 1998 Employer: Hoffmann-La Roche Title: Research assistant Department: Pharma Division Responsibilities: Expert performs R&D on fluorescence techniques and labeling of recombinant proteins (tumor necrosis factor, interferon…) and develops a fluorescence linked immuno binding assays for quality control analysis of biotechnological products.
Expert works under Good Manipulating Practices and Good Laboratory Practices (GMP/GLP).

International Experience

Years Country / Region Summary
Years: 1999 to 2007 Country / Region: USA Summary: The expert was a research assistant at the Lawrence Berkeley National Laboratory, at the University of California in Berkeley and in Los Angeles.
Years: 1997 to 1998 Country / Region: Switzerland Summary: The expert was a research assistant in Hoffman-La Roche in Basel

Career Accomplishments

Associations / Societies
The Biophysical Society
Professional Appointments
Marie-Curie Post-doctoral Fellow;
EMBO Post-doctoral Fellow
Awards / Recognition
Marie-Curie Intra-European Research Fellowship Award;
EMBO Long-Term Research Fellowship Award;
Best PhD Dissertation Award, UCLA Department of Biochemistry;
Keystone Symposia Scholarship;
EMBO Short-Term Fellowship Award;
International Union of Pure and Applied Biophysics Travel Fellowship;
Graduate Award for Excellence in Research UCLA Department of Chemistry and Biochemistry
Publications and Patents Summary
Expert has 22 publications, 1 recent book on nanotechnology, 2 granted patents and 1 patent application

Language Skills

Language Proficiency
English Fluent
French Fluent
German Intermediate

Fields of Expertise

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