Expert in DNA Technology, PCR, Human Identification, Diagnostics, R&D, Technical Support, Management
He worked as a field technical systems specialist for Roche Diagnostics for over 6 years and has expertise in troubleshooting, training and sales support. Expert is familiar with many different technologies and companies involved in the molecular diagnostics field. He has personally visited over 50 leading reference and hospital testing laboratories in the United States.
Expert has over 25 years of academic and corporate experience with different DNA techniques and companies producing products for these applications. These range from nucleic acid isolation, purification, quantitation, amplification, detection and statistical analysis. He considers himself primarily a research scientist with a broad range of experience in the DNA field.
He has worked with PCR and other DNA amplification techniques almost since their inception. He has used these methods in a number of applications and published peer-reviewed articles describing this research. Expert is familiar with many variations of PCR including its use in human identification, real-time fluorescent PCR, use of Locked Nucliec acid (LNA) primers, amplification from RNA targets and many other variations.
He has experience in DNA analysis in several areas, especially in human identification and molecular diagnostics for infectious disease testing. He has worked with DNA quality control processes and documentation as applicable to AABB criteria.
Expert has developed DNA-based assays on many occasions with several biotechnology companies. He has also done extensive troubleshooting and technical support of assays. This expert has utilized DNA kits and products from a wide range of vendors so has a wide and extensive experience base. He is familiar with many different assay formats, including probe-based, homogeneous, and real-time fluorescent PCR.
He has worked with DNA fingerprinting since its beginnings and seen the technology change and evolve over time. This includes the changes in methods, validation and even DNA targets from the initial research through the current CODIS standards. The methodology and basis of the techniques are extremely familiar to this individual, who has worked from bench scientist to Lab Director levels with these techniques.
Expert has worked with DNA technology for over 25 years in both academic and corporate settings. He has also established his own corporation with this being the primary focus of this enterprise. He has worked with many different technical platforms and different applications with DNA technology as the central theme. He has a strong publication record related to human identification and DNA research.
He has utilized the state-of the art genetic sequencing instrumentation and reagents during recent work both in R&D and parentage testing. He is very familiar with the subtle differences in results generated by sequencing systems and optimization of DNA sequence output. He also has bioinformatics experience for sequence data interpretation and utilization. He knows the differences between in-house DNA squencing at low and high throughput, as well as leading service providers in this area.
This expert has extensive experience in developing and utilizing state of the art genotyping systems. He has compared results with various methods and is comfortable with the theory and practice of these methods in various applications. He is familiar with state of the art as well as past methods utilized in this application with both a hands-on and theoretical background.
Expert has served as Laboratory Director for three labs in the human identification field using DNA-based methods since 2000. He has worked under AABB regulations and been a vital link in the success of these organizations. He also has published work on the basis of these methods and has experience with the most current techniques and companies involved in human relationship (parentage, sibling, grandparentage, etc.) and DNA forensics testing.
He has spent over 25 years in academic and corporate settings utilizing emerging molecular biology tools and techniques. He has a very broad range of experience and can answer a wide range of technical and market-based questions related to the field of molecular biology and DNA technology in general. One of his great strengths is being able to communicate the message to the appropriate scientific level of the target audience.
He has extensive molecular diagnostics experience obtained while performing a field technical support role for Roche Diagnostics during the mid to late 1990s. He is familiar with the methodology, regulatory environment and corporate competition in this field, as well as the location and abilities of the client laboratories comprising the end-users of nucleic acid diagnostic testing products and equipment.
Expert has worked with PCR for many years in several different roles with a number of different organizations. He has optimized many systems, assessed and supported many others during the nearly 20 years since he has used the technique. He is experienced and familiar with the theory and practice of older gel-based detection technology right through to leading-edge quantitative real-time PCR and competing technologies. Expert has published several peer-reviewed journal articles as lead author on advancement of PCR methods in the past 10 years.
He is Laboratory Director for two human parentage/relationship testing labs, including oversight of all AABB-accredited procedures and processes, sign-off on all final reports and participation in on-site audits.He has provided scientific consulting services to clients such as feasibility of business planning and laboratory space design and optimization.He has provided marketing and sales support for biotech and diagnostic products and services, along with development of his own consulting business after serving as an independent contractor.
Expert may consult nationally and internationally, and is also local to the following cities: Denver, Colorado - Colorado Springs, Colorado - Aurora, Colorado - Fort Collins, Colorado - Arvada, Colorado - Westminster, Colorado - Boulder, Colorado - Greeley, Colorado - Longmont, Colorado - Cheyenne, Wyoming
|Year: 1998||Degree: PhD||Subject: Biology/Zoology||Institution: Colorado State University|
|Year: 1987||Degree: MS||Subject: Microbiology||Institution: Clemson University|
|Year: 1984||Degree: BS||Subject: Biochemistry||Institution: Clemson University|
|Year: 1981||Degree: AS||Subject: Math/Science||Institution: Somerset County College|
|Years: 2006 to Present||Employer: Undisclosed||Title: owner/CEO||Department: (Undisclosed)||Responsibilities: Management and execution of scientific consulting services, including Laboratory Director for two human relationship testing companies.|
|Years: 2003 to Present||Employer: Undisclosed||Title: Laboratory Director||Department: Relationship Testing||Responsibilities: Laboratory Director responsible for processes and test results for DNA-based parentage testing following AABB regulations.|
|Years: 2005 to Present||Employer: Undisclosed||Title: Laboratory Director||Department: Relationship Testing||Responsibilities: Laboratory Director responsible for processes and test results for DNA-based parentage testing in the Dominican Republic following AABB regulations.|
|Years: 2003 to 2006||Employer: (Undisclosed)||Title: Group Leader||Department: R&D||Responsibilities: Design and implement experiments for myself and 2-5 junior-level scientists for nucleic acid isolation and amplification products.|
|Years: 2000 to 2003||Employer: (Undisclosed)||Title: Senior Scientist||Department: R & D||Responsibilities: Experimental design, execution and management of junior scientist exploring LNA applications. Work and collaboration yielded 4 publications in peer-reviewed journals.|
|Years: 1999 to 2000||Employer: (Undisclosed)||Title: Senior Applications Scientist||Department: (Undisclosed)||Responsibilities: Developed and supported applications for bioinformatics tools for the pharmaceutical and biotechnology sectors.|
|Years: 1993 to 1998||Employer: (Undisclosed)||Title: Senior Field Technical Support Specialist||Department: AMPLICOR PCR Diagnostics||Responsibilities: Supported client clinical/reference labs throughout the US using PCR kits and instrumentation for infectious disease diagnostics. Provided troubleshooting and training both on-site and through various communication channels for FDA-cleared and pending AMPLICOR product line.|
|Years: 1990 to 2003||Employer: (Undisclosed)||Title: Senior Scientist, Lab Director||Department: R&D, Parentage testing||Responsibilities: Served twice with AGTC, Inc.: intiially in R&D of human and animal genetic identification systems and later (2000-2003) as consulting Laboratory Director responsible for all paternity testing work.|
|Years||Country / Region||Summary|
|Years: to Present||Country / Region: Dominican Republic||Summary: He is acting Laboratory Director for Relationship testing in the only AABB-accredited DNA laboratory in the country and south of the USA.|
|Associations / Societies|
|Publications and Patents Summary|
|He has 8 publications as lead or co-author in peer-reviewed journals on topics of LNA applications and human & animal genetic identification.|
|Expert Witness Experience|
|Expert has previously consulted with a 3-4 law firms several years back on DNA testing evidence evaluation. More information available upon request.|
|Training / Seminars|
|He has performed numerous DNA technology training workshops, including several in the past year focused on human identification which including expert lecture and lab services.|
|He has located, tested and validated equipment and reagents specific to particular vendors in the DNA purification, amplification and detection areas.|
|He has worked closely with marketing colleagues at several companies in bringing products to market and supporting those with training and troubleshooting once in the field.|
|Other Relevant Experience|
|He has worked under FDA and AABB regulatory guidelines in product support and services work. He has also established his own company and is familiar with the business start-up and establishment processes.|
|Spanish||Primary language used in the Dominican Republic|