Expert Details

Expert in Flow Cytometry

Expert ID: 730242

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Expert has been in the lab over 20 years focusing in oncology and immunology. She has been involved in assay development utilizing ELISAs, Westerns, ELISpot and flow cytometry to forward multiple research projects. Her area of expertise is in flow cytometry and cell sorting. She has been teaching other colleagues in the application of flow cytometry in their research. This training includes formal classroom education as well as hands-on training. She has been the "go-to" person for all flow cytometry assay issues at Pfizer for the past 4 years. She has setup flow cytometry core facilities, saving time, space, and money. This gave many other labs access to flow cytometry to benefit their research efforts.

She was a consultant to all labs at Pfizer in all their flow cytometry needs.She is currently consulting with a CRO to validate a flow cytometry assay for diagnosis of leukemia and lymphoma.She is currently consulting with a company to edit their current online coursework and assist in developing new coursework.

Expert may consult nationally and internationally, and is also local to the following cities: Los Angeles, California - San Diego, California - Long Beach, California - Santa Ana, California - Anaheim, California - Riverside, California - Glendale, California - Huntington Beach, California - San Bernardino, California - Chula Vista, California

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Year Degree Subject Institution
Year: 1992 Degree: BS Subject: Molecular, Cellular, Developmental Biology Institution: University of Colorado -Boulder
Year: 1992 Degree: BS Subject: Biochemistry Institution: University of Colorado -Boulder

Work History

Years Employer Title Department Responsibilities
Years: 2007 to 2011 Employer: Pfizer Title: Sr Scientist Department: Responsibilities: • Consulted with research teams to setup flow cytometry assays, perform acquisition on digital and analog machines, analyze and interpret data
• Employed flow cytometry in the investigation of cell cycle arrest and apoptosis of cells in support of cancer drug development
• Established flow cytometry core facility, organizing equipment throughout campus to allow for better maintenance and usage of the machines, saving space for other groups and instruments
• Performed cell sorting using FACS Aria I of cancer stem cells for mammosphere assays
• Identified blood donors expressing biomarkers as positive controls for clinical assays
• Developed phagocytosis assay to support mechanism of action for an IND filing
• Identified biomarkers to isolate cancer stem cells from xenograft tumors
• Managed interdepartmental flow cytometry education programs, conducting classroom and hands-on training
Years: 2005 to 2007 Employer: Arena Pharmaceuticals Title: Sr Research Assoc Department: Responsibilities: • Built new multicolor flow cytometry lab with high throughput capability
• Designed and developed platelet activation studies using flow cytometry
• Created novel flow cytometry assay to monitor reactive oxygen species (ROS) in drug treated granulocytes
• Isolated and monitored stem cells for phenotype changes after drug stimulation
• Characterized orphan receptors in cell lines and blood donors
Years: 2003 to 2005 Employer: CancerVax Title: Sr Research Assoc Department: Responsibilities: • Developed clinical assays to screen patient immune responses to vaccine using ELISAs, T cell assays and complement assays
• Discovered method for distinguishing multiple cell lines within the vaccine as a quantitative composition assay
• Supervised and trained RA in ELISAs, T cell assays, and flow cytometry assays
• Authored technical reports and SOPs for clinical trials
Years: 2002 to 2003 Employer: Agensys Title: Sr Research Assoc Department: Responsibilities: • Designed and developed functional assays to characterize new mouse and human MAbs using flow cytometry
• Provided an understanding of involvement of genes using transient and stable tranfections
• Performed phosphorylation assays using ELISAs and westerns
• Employed flow cytometry in the study of apoptosis
Years: 1999 to 2002 Employer: Coulter Pharmaceuticals/Corixa Title: Sr Research Assoc Department: Responsibilities: • Performed 51Cr release assays to understand the ability of a humanized CD20 antibody to induce ADCC in support of clinical trials
• Conducted robust radioassays to determine the antibody Kd
• Developed internalization assays using radioisotopes and toxins
• Created a capillary tube formation assay for determination of effects on angiogenesis
• Utilized flow cytometry and fluorescent microscopy to study antibody binding
• Setup human and primate blood cytokine assays
• Coordinated blood donation program
Years: 1997 to 1998 Employer: Anergen Title: Sr Research Assoc Department: Responsibilities: • Developed ELISpot assay to monitor cytokine profiles of patients during treatment
• Performed intracellular flow cytometry
• Designed an in-situ RT-PCR assay as an alternative to monitor T cell reactivity
• Transferred islets into a diabetic mouse model
• Initiated in-house blood donor program
Years: 1993 to 1997 Employer: Genzyme Title: Research Assoc Department: Responsibilities: • Designed antibody phage display libraries to select fetal cells from maternal blood
• Created cDNA phage libraries for antigen identification / epitope mapping
• Utilized flow cytometry and fluorescent in-situ hybridization (FISH) to determine specific binding of fetal specific antibodies
• Characterized antibodies using flow cytometry and immunoprecipitation
Years: 1992 to 1992 Employer: Sterling Winthrop Title: Research Scientist Department: Responsibilities: • Designed peptide phage display libraries for antigen identification / epitope mapping
• DNA sequencing
• Antibody purification and biotinylation
• Antibody characterization using ELISA

Additional Experience

Training / Seminars
Flow cytometry education, classroom and hands-on
Vendor Selection
Researched and recommended new equipment and antibody sources for companies.
Other Relevant Experience
Personalized training from the beginner to the more advanced. Very personable and in depth for all levels of experience.

Language Skills

Language Proficiency
English She is very proficient in her native language of English. She is involved in consulting with a company in editing their online coursework.

Fields of Expertise

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