Expert Details
Flow Cytometry
ID: 730242
She was a consultant to all labs at Pfizer in all their flow cytometry needs.She is currently consulting with a CRO to validate a flow cytometry assay for diagnosis of leukemia and lymphoma.She is currently consulting with a company to edit their current online coursework and assist in developing new coursework.
Education
| Year | Degree | Subject | Institution |
|---|---|---|---|
| Year: 1992 | Degree: BS | Subject: Molecular, Cellular, Developmental Biology | Institution: University of Colorado -Boulder |
| Year: 1992 | Degree: BS | Subject: Biochemistry | Institution: University of Colorado -Boulder |
Work History
| Years | Employer | Title | Department |
|---|---|---|---|
| Years: 2007 to 2011 | Employer: Pfizer | Title: Sr Scientist | Department: |
Responsibilities:• Consulted with research teams to setup flow cytometry assays, perform acquisition on digital and analog machines, analyze and interpret data• Employed flow cytometry in the investigation of cell cycle arrest and apoptosis of cells in support of cancer drug development • Established flow cytometry core facility, organizing equipment throughout campus to allow for better maintenance and usage of the machines, saving space for other groups and instruments • Performed cell sorting using FACS Aria I of cancer stem cells for mammosphere assays • Identified blood donors expressing biomarkers as positive controls for clinical assays • Developed phagocytosis assay to support mechanism of action for an IND filing • Identified biomarkers to isolate cancer stem cells from xenograft tumors • Managed interdepartmental flow cytometry education programs, conducting classroom and hands-on training |
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| Years | Employer | Title | Department |
| Years: 2005 to 2007 | Employer: Arena Pharmaceuticals | Title: Sr Research Assoc | Department: |
Responsibilities:• Built new multicolor flow cytometry lab with high throughput capability• Designed and developed platelet activation studies using flow cytometry • Created novel flow cytometry assay to monitor reactive oxygen species (ROS) in drug treated granulocytes • Isolated and monitored stem cells for phenotype changes after drug stimulation • Characterized orphan receptors in cell lines and blood donors |
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| Years | Employer | Title | Department |
| Years: 2003 to 2005 | Employer: CancerVax | Title: Sr Research Assoc | Department: |
Responsibilities:• Developed clinical assays to screen patient immune responses to vaccine using ELISAs, T cell assays and complement assays• Discovered method for distinguishing multiple cell lines within the vaccine as a quantitative composition assay • Supervised and trained RA in ELISAs, T cell assays, and flow cytometry assays • Authored technical reports and SOPs for clinical trials |
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| Years | Employer | Title | Department |
| Years: 2002 to 2003 | Employer: Agensys | Title: Sr Research Assoc | Department: |
Responsibilities:• Designed and developed functional assays to characterize new mouse and human MAbs using flow cytometry• Provided an understanding of involvement of genes using transient and stable tranfections • Performed phosphorylation assays using ELISAs and westerns • Employed flow cytometry in the study of apoptosis |
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| Years | Employer | Title | Department |
| Years: 1999 to 2002 | Employer: Coulter Pharmaceuticals/Corixa | Title: Sr Research Assoc | Department: |
Responsibilities:• Performed 51Cr release assays to understand the ability of a humanized CD20 antibody to induce ADCC in support of clinical trials• Conducted robust radioassays to determine the antibody Kd • Developed internalization assays using radioisotopes and toxins • Created a capillary tube formation assay for determination of effects on angiogenesis • Utilized flow cytometry and fluorescent microscopy to study antibody binding • Setup human and primate blood cytokine assays • Coordinated blood donation program |
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| Years | Employer | Title | Department |
| Years: 1997 to 1998 | Employer: Anergen | Title: Sr Research Assoc | Department: |
Responsibilities:• Developed ELISpot assay to monitor cytokine profiles of patients during treatment• Performed intracellular flow cytometry • Designed an in-situ RT-PCR assay as an alternative to monitor T cell reactivity • Transferred islets into a diabetic mouse model • Initiated in-house blood donor program |
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| Years | Employer | Title | Department |
| Years: 1993 to 1997 | Employer: Genzyme | Title: Research Assoc | Department: |
Responsibilities:• Designed antibody phage display libraries to select fetal cells from maternal blood• Created cDNA phage libraries for antigen identification / epitope mapping • Utilized flow cytometry and fluorescent in-situ hybridization (FISH) to determine specific binding of fetal specific antibodies • Characterized antibodies using flow cytometry and immunoprecipitation |
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| Years | Employer | Title | Department |
| Years: 1992 to 1992 | Employer: Sterling Winthrop | Title: Research Scientist | Department: |
Responsibilities:• Designed peptide phage display libraries for antigen identification / epitope mapping• DNA sequencing • Antibody purification and biotinylation • Antibody characterization using ELISA |
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Additional Experience
| Training / Seminars |
|---|
| Flow cytometry education, classroom and hands-on |
| Vendor Selection |
|---|
| Researched and recommended new equipment and antibody sources for companies. |
| Other Relevant Experience |
|---|
| Personalized training from the beginner to the more advanced. Very personable and in depth for all levels of experience. |
Language Skills
| Language | Proficiency |
|---|---|
| English | She is very proficient in her native language of English. She is involved in consulting with a company in editing their online coursework. |