Expert Details

Expert in Mass Spectrometry, HPLC/MS, Ion Trap MS, LC/MS/MS in Environmental, Food, Proteomic, and Pharmaceutical Analysis

Expert ID: 720719 North Carolina, USA

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Expert has been hired by law firms as an expert witness in the area of analytical chemistry. He has worked with 15 law firms in his career, with some of the cases going over multiple years. This experience includes:
• Preparation of 20 expert reports.
• Appearing at 10 depositions.
• Testifying in court three separate occasions.
• Performing laboratory experiments in my laboratory to support a case for three different clients.
Background and experience is ideal for support cases involving:
• Environmental cases
• Pharmaceutical and biotechnology cases.
• Cases that need additional laboratory experiments to generate data to support the case.
• Patent infringement
• Mass spectrometry measurements

Expert has been responsible for developing mass spectroscopic methods for use on environmentally and biologically significant compounds. While he has employed HPLC/MS and CE/MS on quadrupoles, sectors and ion trap mass spectrometers for positive and negative ion detection of specific compounds, Expert has extensive experience in analyzing environmentally and biologically significant compounds using other methods. These include direct probe MS, gas chromatography/high-resolution mass spectrometry (GC/HRMS), and MS/MS. GC/MS analysis has been used for the determination of volatile organic compounds desorbed from cartridges (e.g. tenax) semivolatile organics in the environment (e.g. PAH's, PCB, TCDD, and pesticides) and pharmaceuticals. Expert is experienced in EI, CI, and NCI techniques using low resolution, high resolution, and tandem mass analyzers. His research in mass spectrometry has resulted in over 230 publications and presentations.

Expert has worked on the development and evaluation of electrospray HPLC/MS for the analysis of high molecular weight opioid peptides at the femtomolar level, as well as the analysis of cyclodextrins to correlate chemical modification of the structure with binding to organophosphate poisons. He has also worked on the development of electrospray HPLC/MS methods to insure food safety, and has evaluated the use of electrospray HPLC/MS to determine compounds of environmental interest. These include dyes, pesticides, and herbicides. He currently has four electrospray HPLC/MS systems in the laboratory. This experience ranges from impurity and metabolite identification to quantitative analysis of target drugs for pharmacokinetic studies.

Expert's pioneering work in HPLC/MS has demonstrated that this method can be used to perform analyses of compounds not amenable to analysis by GC/MS or MS/MS. He developed a research program around HPLC/MS and HPLC/MS/MS using electrospray, thermospray and particle beam interfaces. He investigated fundamental principles of the applications of these techniques to current analytical problems. He has used HPLC/MS for the analysis of EPA Appendix VIII compounds, dyes, pesticides, herbicides, numerous drugs and their metabolites of pharmaceutical interest (e.g., AIDS drugs, anticancer drugs, antimalarials), mycotoxins and their metabolites, natural products, peptides, nucleotides, and proteins. He has seven HPLC/MS interfaces: two thermospray, one particle beam and four electrospray. He has over 230 publications and presentations in the area of HPLC/MS.


For the past nine years, Expert has been working with FDA, developing new, more specific and more sensitive methods to detect trace residues in food products. His work primarily used LC/MS, GC/MS, and LC methods to detect antibiotics and common veterinary drugs in foods as well as pesticides and herbicides in food products. Currently Expert is working on technological advancements in LC/MS, researching novel ways to detect and quantitate food allergens based on proteomic approaches, He is working with clients in the area of protein marker discovery, protein characterization and protein quantitation. Additionally, he has developed novel procedures to detect food allergens and pharmaceutical products in manufacturing facilities to insure minimal cross contamination in consumer products.

Expert has been using mass spectrometry for the past 25 years to perform trace analyses in biological and environmental media. He has worked with numerous pharmaceutical companies, FDA, EPA, and the National Institute of Drug Abuse in developing and validating methods as well as analyzing samples in the 0.1 pg/mL to 1ng/mL range.

Expert gas used LC high resolution MS/MS to characterize and identify proteins via enzymatic digestion digestion followed LC/MS/MS and database searching and by peptide sequencing to identify proteins and to quantitate proteins. Also he has analyzed intact proteins to determine stability with time under various test conditions as well as activity via an affinity assay.
• Developed new methodologies for detection of food allergens using proteomics and LC/MS/MS.
• Developed novel tagging approached to determine allergenic gluten peptides from digestion.
• Measured several different monoclonal antibodies
• Identified proteins excreted through human kidney exosomes.

Expert has presented over 50 on-site training classes on LC/MS. He has conducted on-site method development and instrument training to meet clients' objectives. He has performed an on-site review of an analytical chemistry program. He conducted an on-site review of LC/MS results and methods. Provided expert review of data and generated data to support clients claims in court cases.

Expert may consult nationally and internationally, and is also local to the following cities: Raleigh, North Carolina - Greensboro, North Carolina - Durham, North Carolina - Winston Salem, North Carolina - Fayetteville, North Carolina - Cary, North Carolina - High Point, North Carolina - Greenville, North Carolina - Concord, North Carolina - Lynchburg, Virginia

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Education

Year Degree Subject Institution
Year: 1982 Degree: Ph.D. Subject: Analytical Chemistry Institution: University of North Carolina
Year: 1978 Degree: B.S. Subject: Chemistry Institution: Canisius College, Buffalo, N.Y.

Work History

Years Employer Title Department Responsibilities
Years: 2001 to Present Employer: Undisclosed Title: President Department: Mass Spec Consulting Responsibilities: Expert oversees operation of technical services in mass spectrometry, their training program, and development of new products and services.
Years: 1983 to 2001 Employer: Research Triangle Institute Title: Senior Research Mass Spectrometrist Department: Analytical & Chemical Sciences Responsibilities:

International Experience

Years Country / Region Summary
Years: to Present Country / Region: Europe Summary: Expert conducted seminar tours and hands-on training on LC/MS.
Years: to Present Country / Region: Asia Summary: He conducted seminar tours and hands on training on LC/MS.

Career Accomplishments

Associations / Societies
Expert is a member of the American Chemical Society and the American Society for Mass Spectrometry.

Professional Societies, Awards, and Honors
• Organizer of the (4 years undisclosed) Montreux LC/MS Symposium
• Appointed Adjunct Professor at the University of North Carolina School of Pharmacy
• Member of the Editorial Board for the Journal of Chromatography
• Received “The RTI Presidents Award” the highest award given at RTI for leveraging RTI’s scientific reputation to acquire state-of-the-art equipment in the pursuit of Analytical Chemistry
• Organizer of the ASMS Symposium on LC/MS Methods, and Instrumentation
• Elected to the Office of "Member at Large - Standards and Measurements" to the American Society for Mass Spectrometry
• Co-editor of a special series on LC/MS to be published by the Journal of Chromatography
• Member of Coordinating Committee for the Montreux LC/MS Symposium Series
• Organizer of the 12th Montreux Symposium on Liquid Chromatography/Mass Spectrometry at Hilton Head Island, SC
• Member of the A-page advisory panel for Analytical Chemistry
• Co-organizer of the 7th Sanibel Conference on Mass Spectrometry, Sanibel Island, on The Physics and Chemistry of Electrospray and Multiple Charge Ions.
• Co-organized ACS Mini-Conference on "Advances in Mass Spectrometry", Raleigh, NC
• Co-organizer of "International Montreux Conference on LC/MS" at Cornell
• Editorial Advisory Board for Series "Modern Mass Spectrometry", CRC Press,
• Invited to organize and chair LC/MS symposium to be held at the FACSS Meeting
• Philadelphia, PA, September 1992.
• Organized and chaired symposium of LC/MS for Nonvolatile Pollutants for the 38th Annual American Society for Mass Spectrometry and Allied Topics
• Co-chairman of the Triangle Mass Spectrometry Discussion Group 1990 1992.
• Received RTI's Professional Development Award to write a book on the Theory and Applications of HPLC/MS.
• Adjunct Associate Professor at North Carolina State University, Department of
• Anatomy, Physiological Sciences and Radiology (1986 present).
• Served as a reviewer for articles to Analytical Chemistry, Rapid Communications in
• Mass Spectrometry, Biological Mass Spectrometry, J. Am. Soc. for Mass Spectrometry, JAOAC, Journal of Chromatography, and for proposals to EPA, NSF, NRC, and North Carolina Board of Science and Technology.
• Chairman of the LC/MS Oral Session for the Annual Conference on Mass Spectrometry and Allied Topics
• Chairman of the LC/MS Poster Session for the 33rd Annual Conference on Mass
• Spectrometry and Allied Topics, San Diego, CA
• American Chemical Society
• American Society for Mass Spectrometry Conference Fellowship
• American Society for Mass Spectrometry
• Merck Index Award for Outstanding Undergraduate Analytical Chemist
Professional Appointments
Expert is Adjunct Associate Professor at a State University, Department of Anatomy, Physiological Sciences and Radiology and an Adjunct Professor at a university's School of Pharmacy. He reviews proposals submitted to the EPA, NSF, and the Board of Science and Technology as well as articles submitted to several journals including Analytical Chemistry, Biological Mass Spectrometry, Journal of the American Society for Mass Spectrometry, and the Journal of Chromatography. He has chaired national conferences on Mass Spectrometry. He served as an American Society for Mass Spectrometry board member "member-at-large for measurement and standards." He has organized and hosted International Montreux LC/MS Symposium held at Hilton Head Island, and organized the Montreux symposium. He was a co-organizer for the Sanibel Conference on Mass Spectrometry on the Physics and Chemistry of Electrospray and Multiple Charged Ions. He also serves as an "A-page" advisor for Analytical Chemistry and is on the Editorial Advisory Board for the Press Series "Modern Mass Spectrometry."
Awards / Recognition
He received the RTI President's Award for securing new equipment for industry in the laboratory.
Publications and Patents Summary
Expert has written over 300 publications and presentations.

Over 250 presentations at scientific meetings:
• 83 Invited lectures
• 19 keynote lectures
• 5 plenary lectures

Additional Experience

Expert Witness Experience
Expert has served as an expert witness to support analytical findings or to generate analytical data to support a case. He has been involved in nine different cases, provided six depositions and was on the stand in three different court cases.
Training / Seminars
Expert has been a visiting lecturer on numerous occasions nationally. For the past 10 years, he has been conducting international training and custom short courses in mass spectrometry. These courses include one-, two-, and four-day sessions on liquid chromatography/mass spectrometry (LC/MS), gas chromatography/mass spectrometry (GC/MS), and mass spectral interpretation. He has taught single-day courses on GC, LC, and capillary electrophoresis (CE). Expert can design courses to cover specific topics such as pharmaceutical and environmental areas. He can also cover newer topics like ion trap-MS, capillary electrophoresis/MS, electrospray-MS, and sample preparation. In addition, he has provided instrument-specific MS training for Hewlett Packard, with Bruker and PE Sciex training planned for 1998. Expert has delivered these courses for a number of clients including the American Society for Mass Spectrometry, over 10 pharmaceutical companies, ISSX, separation focused meetings (HPCE, HPLC), industry, and international government or industrial supported education and training programs.
Vendor Selection
He has experience locating vendors in the area of mass spectrometry, including GC/MS, LC/MS, electrospray mass spectrometry, thermospray LC/MS, and particle beam LC/MS.

He has access to printed materials and personal contacts for assistance in vendor location. The eight mass spectrometers in his lab represent most manufacturers and his experience with these instruments can prove valuable to a potential buyer.

Fields of Expertise

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