Expert in Metabolomics, Mass Spectrometry and Plant Pathology
Expert ID: 729431 United Kingdom
Expert has been running non-targeted and targeted metabolomics fingerprinting experiments from the plant samples from the past three years and has a vast experience of running various instruments including LTQ FIE-MS, FT-ICR-MS, HPLC-PDA, LC-MS-PDA, GC-MS, MALDI-TOF-MS, FTIR, as well as MSn fragmentation both in FT and ion-trap modes. He has developed new protocols for extraction of particular classes of compounds from various plant samples (such as leaves, stems, cell wall, etc) as well as overall metabolites extraction. He routinely use highly reliable methods to validate sampling methodologies using unsupervised and supervised multivariate data analysis, such as PCA, LDA, Random forest, ANOVA, p-values etc. He has a great deal of experience in handling MS data and interpretation of the MS results, investigation of explanatory signals and accurate mass matrix analysis by database searches at high mass accuracy (1ppm) and comparing fragmentation patterns as well as comparisons with known standards. He has also been quantitatively measuring soluble phenolics and phenolics conjugates, phenyl propanoids, aromatic amino acids and flavonoids by HPLC, LC-MS and GC-MS to quantify lignin precursors and flavonoids involved in plant defence. He has also experience of reverse-phase chromatographic seperation of compounds and preparation of samples for NMR analysis.
Expert has indepth knowledge of peptide mass fingerprinting and metabolomics. He has a degree in Bioinformatics too, and has a good programming ability using C-language, PERL (Including, database handling, applications development, providing web interface to PERL programs, and generating real time graphics using GNU plot), JAVA and HTML. He has a good knowledge and use of various algorithms, and reasonable understanding of UNIX and GCG package. He can manage various biological databases using SQL, Microsoft Access and has been part of construction and validation of MZedDB (Tool for the annotation of High Resolution MS metabolomics data). He has experience of using various Bioinformatics related softwares, such as, PrimerSelect, MegAlign, Protean, Rasmol and Swiss-PDB viewer, and peptide mass spectrometric related softwares, such as Voyager-DE Instrument Control, Data Explorer and Biocypher. He has developed in house microbial peptide mass spectrometric fingerprinting database and have experties in rapid identification of unknown microorganisms by MALDI-TOF- mass spectrometry.
|Year: 2007||Degree: MSc||Subject: Bioinformatics||Institution: Sheffield Hallam University, UK|
|Year:||Degree: PhD (Will complete in 2011)||Subject: Plant Pathology and Metabolomics||Institution: Aberystwyth University, UK|
|Year: 2004||Degree: M.Phil||Subject: Biochemistry and Molecular Biology||Institution: Quaid-I-Azam University, Pakistan|
|Year: 2001||Degree: MSc||Subject: Biochemistry||Institution: University of Agriculture, Pakistan|
|Year: 1999||Degree: BSc||Subject: Chemistry||Institution: The University of Punjab, Pakistan|
|Years: 2007 to Present||Employer: Undisclosed||Title: Research Assistant||Department: Institute of Biological, Environmental and Rural Sciences||Responsibilities: Expert has embarked on a project to study metabolic reprogramming during pathogen challenged plants using various mass spectrometric, biochemical and enzymatic analysis techniques. His main job responsibility is to prepare and run various samples (i.e drought, cold, pathogen stressed plant samples, etc) on broad range of mass spectrometers including LTQ-FIE MS, LTQ- FT-ICR MS, GC-MS, HPLC PDA, Exactive-LC MS, LTQ-LC-MS, FT-IR, etc, and then analyse and interpret the results elucidating the metabolic pathways affected by abiotic and biotic plant stresses.|
|Years: 2003 to 2003||Employer: Nuclear Institute of Agriculture and Biology, Pakistan||Title: Research Assistant||Department: Biological Chemistry Division||Responsibilities: Expert has been working on a project involving mutagenic improvement of locally isolated bacteria for amino acids production utilizing industrial waste products. His responsibilities included screening of locally isolated bacteria, amino acid fermentation utilising industrial and agricultural bi-products, and then analogue resistant mutant screening for enhanced production of lycine.|
|Years: 2003 to 2003||Agency: Nuclear Institute of Agriculture and Biology, Pakistan||Role: Research Assistant||Description: Expert was engaged on Pakistan Science Foundation project on mutagenic improvement of locally isolated bacteria by utilizing industrial waste products.|
|Associations / Societies|
|Publications and Patents Summary|
|He has 12 Publications. A book chapter is accepted for publication in Annual Plant Reviews, Biology of Plant Metabolomics, which will publish in April 2011.
Fields of Expertise
agriculture, biochemistry, host resistance, pathogenesis, pathology, biology, analytical biochemistry, cryopreservation, electrospray ionization, Fourier transform mass spectroscopy, high-performance liquid chromatography/mass spectroscopy, ion cyclotron resonance mass spectroscopy, ion trap mass spectrometry, liquid chromatography/mass spectroscopy, mass spectrometry, tandem mass spectrometry, metabolomics, bioinformatics, computational biology, protein mass spectrometry, chromatography applications, Perl programming language, database technology, cation-exchange chromatography, metabolite, gas chromatography/mass spectrometry, amino acid metabolism, deionized water, enzyme purification, plant biochemistry, chromatography data analysis, chromatography method development, atomic absorption spectroscopy, high-performance liquid chromatography, enzyme technology, DNA, amino acid, microbiology, Fourier transform infrared spectroscopy, Fourier transform, enzymology, botany