Expert Details

Expert in Metabolomics, Mass Spectrometry and Plant Pathology

Expert ID: 729431 United Kingdom

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Expert has developed expertise in metabolic reprogramming during susceptible and resistant interactions of Magnaporthe grisea with host plants, Brachypodium distachyon and rice as well as abiotic stress resistance in plants such as drought or cold tolerance. Such systems present many challenges for both sampling and data alignment and thus Expert has developed and validated new methods for efficient handling of the plant-pathosystem including synchronized and reproducible infection, sampling and monitoring biological and metabolic changes in the most destructive rice blast disease. He has found novel biochemical mechanisms responsible for fungal pathogenesity and mechanisms responsible for host resistance in rice blast disease thus contributed greatly in the field of agriculture. His has developed new expertise in the areas of fungal imaging in infected leaves, staining of ROS and in-situ activity determination of certain enzymes, such as NADP-malic enzyme and shikimate dehydrogenase, to elucidate mechanisms responsible for infection and resistance.

Expert has been running non-targeted and targeted metabolomics fingerprinting experiments from the plant samples from the past three years and has a vast experience of running various instruments including LTQ FIE-MS, FT-ICR-MS, HPLC-PDA, LC-MS-PDA, GC-MS, MALDI-TOF-MS, FTIR, as well as MSn fragmentation both in FT and ion-trap modes. He has developed new protocols for extraction of particular classes of compounds from various plant samples (such as leaves, stems, cell wall, etc) as well as overall metabolites extraction. He routinely use highly reliable methods to validate sampling methodologies using unsupervised and supervised multivariate data analysis, such as PCA, LDA, Random forest, ANOVA, p-values etc. He has a great deal of experience in handling MS data and interpretation of the MS results, investigation of explanatory signals and accurate mass matrix analysis by database searches at high mass accuracy (1ppm) and comparing fragmentation patterns as well as comparisons with known standards. He has also been quantitatively measuring soluble phenolics and phenolics conjugates, phenyl propanoids, aromatic amino acids and flavonoids by HPLC, LC-MS and GC-MS to quantify lignin precursors and flavonoids involved in plant defence. He has also experience of reverse-phase chromatographic seperation of compounds and preparation of samples for NMR analysis.

Expert has indepth knowledge of peptide mass fingerprinting and metabolomics. He has a degree in Bioinformatics too, and has a good programming ability using C-language, PERL (Including, database handling, applications development, providing web interface to PERL programs, and generating real time graphics using GNU plot), JAVA and HTML. He has a good knowledge and use of various algorithms, and reasonable understanding of UNIX and GCG package. He can manage various biological databases using SQL, Microsoft Access and has been part of construction and validation of MZedDB (Tool for the annotation of High Resolution MS metabolomics data). He has experience of using various Bioinformatics related softwares, such as, PrimerSelect, MegAlign, Protean, Rasmol and Swiss-PDB viewer, and peptide mass spectrometric related softwares, such as Voyager-DE Instrument Control, Data Explorer and Biocypher. He has developed in house microbial peptide mass spectrometric fingerprinting database and have experties in rapid identification of unknown microorganisms by MALDI-TOF- mass spectrometry.

Education

Year Degree Subject Institution
Year: 2007 Degree: MSc Subject: Bioinformatics Institution: Sheffield Hallam University, UK
Year: Degree: PhD (Will complete in 2011) Subject: Plant Pathology and Metabolomics Institution: Aberystwyth University, UK
Year: 2004 Degree: M.Phil Subject: Biochemistry and Molecular Biology Institution: Quaid-I-Azam University, Pakistan
Year: 2001 Degree: MSc Subject: Biochemistry Institution: University of Agriculture, Pakistan
Year: 1999 Degree: BSc Subject: Chemistry Institution: The University of Punjab, Pakistan

Work History

Years Employer Title Department Responsibilities
Years: 2007 to Present Employer: Undisclosed Title: Research Assistant Department: Institute of Biological, Environmental and Rural Sciences Responsibilities: Expert has embarked on a project to study metabolic reprogramming during pathogen challenged plants using various mass spectrometric, biochemical and enzymatic analysis techniques. His main job responsibility is to prepare and run various samples (i.e drought, cold, pathogen stressed plant samples, etc) on broad range of mass spectrometers including LTQ-FIE MS, LTQ- FT-ICR MS, GC-MS, HPLC PDA, Exactive-LC MS, LTQ-LC-MS, FT-IR, etc, and then analyse and interpret the results elucidating the metabolic pathways affected by abiotic and biotic plant stresses.
Years: 2003 to 2003 Employer: Nuclear Institute of Agriculture and Biology, Pakistan Title: Research Assistant Department: Biological Chemistry Division Responsibilities: Expert has been working on a project involving mutagenic improvement of locally isolated bacteria for amino acids production utilizing industrial waste products. His responsibilities included screening of locally isolated bacteria, amino acid fermentation utilising industrial and agricultural bi-products, and then analogue resistant mutant screening for enhanced production of lycine.

Government Experience

Years Agency Role Description
Years: 2003 to 2003 Agency: Nuclear Institute of Agriculture and Biology, Pakistan Role: Research Assistant Description: Expert was engaged on Pakistan Science Foundation project on mutagenic improvement of locally isolated bacteria by utilizing industrial waste products.

Career Accomplishments

Associations / Societies
Metabolomics Society,
SESCOB
Publications and Patents Summary
He has 12 Publications. A book chapter is accepted for publication in Annual Plant Reviews, Biology of Plant Metabolomics, which will publish in April 2011.

Fields of Expertise

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