Expert in Plant Cell Molecular Biology, Biochemistry, Bioenergy, Algal Biotechnology, Biomass Processing
Expert ID: 730633 United Kingdom
Her technical expertise portfolio includes: molecular biology techniques (gene cloning, genetic engineering, transgenic plants, expression analysis, RNAi screening), recombinant protein expression and purification by a variety of approaches, (SDS/LDS PAGE, DIGE), affinity purification of proteins by FPLC; knowledge of protein chemistry and structure and bioinformatics tools; protein characterization by mass spectrometry and proteomic analysis of complex samples by LC-MS/MS and MALDI-TOF MS; mass spectrometry and MALDI MS imaging; imaging by electron microscopy, confocal microscopy, fluorescence microscopy; carbohydrate chemistry and analytical techniques, enzymatic assays; cell biology (animal and plant cell cultures, flow cytometry), and microbiological techniques.
From 2011, she has taken the challenge of developing new technologies for bio-fuel production in the industrial biotechnology in an industrial environment, which recently resulted in a successful patent application for a novel biomass extraction process. Her current scope of research includes bio-energy and bio-fuels sector, ranging from Generation 1 bio-fuels (corn ethanol plants in the US), to Generation 2 (Anaerobic Digestion for biogas production) and to Generation 3 (algae-derived bio-fuels and valuable products). In parallel, she co-supervises an academic bio-medical research project in one of the leading universities in the UK; continues to be a member of the UK Research Councils Referee Panel, reviewing grant applications; and is a peer reviewer for international journals including PLoS ONE, Plant Journal, New Phytologist, Mol. Biol. Cell, AjPP.
Peer review of grant applications for BBSRCPeer review of grant application for EPSRCPeer review of research articles for for international journals including PLoS ONE, Plant Journal, New Phytologist, Mol. Biol. Cell, AjPP.
|Year: 1994||Degree: PhD||Subject: Plant Biology||Institution: Institute of Plant Physiology, Russian Academy of Sciences|
|Year: 1986||Degree: MSc/BSc||Subject: Plant Biology||Institution: Moscow State University|
|Years: 2011 to 2013||Employer: Pursuit Dynamics plc||Title: Senior Scientist||Department: Product Development||Responsibilities: Working in a capacity of a Scientific Consultant and/or a Project Manager on a number of industrial biotechnology projects related to biofuel production, she had a primary role in initiation and supervision of technology development projects, revision of reports and patent applications, technical feasibility assessments and budget forecasts, and an advisory role for establishing of new contacts with external clients and negotiation of contracts. She obtained EC FP7, ERDF and TSB UK external funding grants for research projects.|
|Years: 2006 to 2010||Employer: University of Reading||Title: Senior Research Fellow||Department: School of Biological Sciences||Responsibilities: Initiated and successfully completed 5 research projects, resulting in publication of 10 scientific papers with high-impact factor. Supervised and trained research staff and students.|
|Years: 2001 to 2006||Employer: John Innes Centre||Title: Senior Research Scientist||Department: Cell and Developmental Biology||Responsibilities: Leading small research team, she completed two research programmes, resulting in publication of 5 scientific papers with high-impact factor; supervised PhD, MSc and foreign exchange students; was a member of departmental interview panel; line manager of research assistants.|
|Years: 1995 to 2001||Employer: University of Wales, Bangor||Title: Postdoctoral Fellow, Research Officer||Department: School of Biological Sciences||Responsibilities: She has carried out original research in carbohydrate metabolism and resource allocation, developed new single-cell techniques, supervised and trained research staff and students, managed international collaborations and established a national-level research network.|
|Years: 1987 to 1995||Employer: Russian Academy of Sciences||Title: Research Scientist||Department: Institute of Plant Physiology||Responsibilities: She has established biophysical research programme on developmental dynamics of turgor pressure, membrane hydraulic conductivity and cell wall volumetric elastic modulus in sugar beet cells, using single-cell pressure probe technique. She has accomplished PhD programme and thesis while on the job.|
|Years||Country / Region||Summary|
|Years: 1987 to 1995||Country / Region: Russia||Summary: She held a position of Research Scientist at the Institute of Plant Physiology, Russian Academy of Sciences and carried research programme focussed on physiology, biochemistry and biophysics of sugar accumulation by sugar beet cells.|
|Years: 1999 to 1999||Country / Region: Germany||Summary: She has been a visiting scientist at the KFA research centre in Julich, evaluating use of stable isotopes for trace analysis of relocation of nutrients in plant tissues.
Later, she had a collaboration project at the University of Wurzburg studying the role of potassium ion channels in regulation of sugar transport/carbohydrate metabolism in plant tissues.
|Years: 1999 to 1999||Country / Region: New Zealand||Summary: She had a collaboration project at the HortResearch Institute, studying long-distance transport of sugars and pioneering single-cell analysis of phloem cell sap in Arabidopsis plants.|
|Years: 2011 to 2011||Country / Region: USA||Summary: She led pilot-scale trials with biomass processing for Gen3 biofuels production.|
|Years: 2012 to 2012||Country / Region: USA||Summary: She has evaluated potential for new technological application for Gen1 biofuels production and provided advice for its optimisation.|
|Associations / Societies|
|Society for Experimental Biology, British Society for Cell Biology, British Society for Mass Spectrometry.|
|Awards / Recognition|
|Research Grants and Awards:
2012 - FP7 EU consortium grant for research on biopolymers from algae. She has negotiated the project agreement and manages the project on behalf of her company.
2011 – LC-KEEP trust (ERDF) grant for research on new technology for Gen3 biofuels.
2011 - British Heart Foundation, received in January 2011, which she received as the Principal Applicant, and currently co-supervises Research Scientist working on the project “Stress-induced post-transcriptional regulation of gene expression by the Exon Junction Complex in cardiac myocytes”.
2010 - British Mass Spectrometry Society – she received grant for a summer student project: "Matrix-Assisted Laser Desorption/Ionisation (MALDI) Mass Spectrometry Imaging of Plant Defence Metabolites".
2010 - Institute of Cardiovascular and Metabolic Research - she receive a Research Development Award for a project "The role of Exon Junction Complex protein eIF4A-III in the early response to hypoxia and mechanical stress in cardiac myocytes and fibroblasts".
2008, 2009, 2010 - University of Reading - research travel grants
2001 - Society for Experimental Biology – grant for a workshop in Portugal.
1999 - The Royal Society (£3,000) – collaboration research grant for visit to New Zealand and Australia
|Publications and Patents Summary|
|She has published 28 peer-reviewed research articles and 7 reviews and book chapters in plant biology and analytical methods, 1 patent on industrial biotechnology application.|
|Russian||Her native language is Russian.|
|German||She can read technical literature in German.|
Fields of Expertise
biochemistry, biology, cell (biology), gene expression, molecular biochemistry, molecular biology, plant biochemistry, plant cell biology, plant molecular biology, applied biochemistry, biorefinery, cell damage, bioanalytical chemistry, plant taxonomy, cell disruption, protein characterization, cell death, protein isolation, electroporation, biofuel, translation, mitosis, ethanol fuel production, protein biology, protein biochemistry, immunofluorescence, biological cell development, eukaryote, eukaryotic molecular biology, aquatic biology, analytical biochemistry, protein post-translational modification, biological cell differentiation, biochemical research, biological waste, biological safety, steam sparging, biomass, gene expression regulation, molecular biology translation, immunochemistry, molecular genetics, histology, cell membrane transport, fermentation, DNA manipulation, protein engineering, nucleotide, biological process, polymerase chain reaction, gene amplification, RNA, protein synthesis, protein purification, microbiology, metabolism, genetics, genetic manipulation, genetic engineering, enzymology, cytology, cloning technique, chromosome, chemical analysis, cell physiology, cell culture, botany, biotechnology, biomass fuel, bioenergy cycle, industrial biochemical process