Expert Details

Expert in Protein aggregation, characterization, purification

Expert ID: 732518 Massachusetts, USA

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SUMMARY
• Innovative protein biophysicist and biochemist with 10+ years of proven expertise in protein biochemistry and biophysics
• Led 150 + projects and worked in collaborative and multidisciplinary teams within academic and industrial set-ups
• Performed and supervised projects involving protein and antibody purification and characterization
• Interacted with teams within and outside the company to prepare statement of work, reports, expanded business opportunities in a one-to-one or team settings
• Prepared cost-benefit analysis on an in-house project to improve efficiency and curb losses in a long run
SKILLS
• Nucleic acids: Isolation of mRNA from mouse brain, isolation of plasmid DNA from E. coli DH5 strain, reverse transcription of mRNA, Polymerase Chain Reaction (PCR), Molecular cloning
• Recombinant proteins: Expression and purification of proteins such as prion protein and its variant, αB-crystallin, α-synuclein, 2-microglobulin, CRABP1 and its variants using bacterial expression system, 15N and 13C isotope labeling of proteins, protein modification using fluorophore labeling, SDS-PAGE, Western blotting, and Blue-Native PAGE
• Protein purification: Affinity chromatography, anion and cation exchange chromatography (CEX), HIC, preparative SEC, AKTA FPLC using custom packed columns including XKs (16, 26 and 50) and BPG100 columns, and Tangential flow filtration.
• Protein analysis: Reversed-phase-, and Size exclusion- HPLC (Agilent), LAL assay, Octet (titer assay)
• Spectroscopy: Nuclear Magnetic Resonance (NMR) (Varian 700), UV-Visible Spectroscopy, Fluorescence Spectroscopy (Anisotropy, FRET), Circular Dichroism (CD), Dynamic Light Scattering (DLS), Time Correlated Single Photon Counting (TCSPC), Isothermal Calorimetry (ITC), Small-angle X-ray Scattering (SAXS), Analytical Ultracentrifugation (AUC)
• Microscopy: Total Internal Reflection Fluorescence Microscopy (TIRF-M), Transmission Electron Microscopy (TEM), Atomic Force Microscopy (AFM)
• Productivity: Microsoft Word, Excel, and Power point, Origin, and SigmaPlot
• Programming: MATLAB, R, Python


Education

Year Degree Subject Institution
Year: 2010 Degree: Ph.D. Subject: Biochemistry Institution: CCMB, India
Year: 2003 Degree: M.Sc. Subject: Life Sciences Institution: JNU, India
Year: 2001 Degree: B.Sc. Subject: Agricultural Sciences Institution: BHU, India

Work History

Years Employer Title Department Responsibilities
Years: 2018 to Present Employer: Undisclosed Title: Senior Purification Scientist Department: Responsibilities: Research
Years: 2016 to 2018 Employer: Title: Senior Scientist- Protein Sciences Department: Responsibilities: • Led team as a project manager
• Team lead-scale-up purification
• Led as a Study Director for a few projects with 10-15 team members
• Performed 150+ projects to their completion in one of the highest performing groups
• Worked in the downstream process development (DSP) such as purification of antibodies and mammalian cells at milligram to gram scales under low endotoxin condition
• Involved in purification and characterization of proteins from bacterial, yeast, insects and mammalian cells.
• Performed IMAC, IEX, affinity including GST, streptactin, softlink, ProA purifications, membrane enrichments using N2 bomb and sonication
• Performed SEC using S400, S200, S75 columns
• Performed large scale purification using BPG100 columns with 1-2 L of resins
• Characterized protein using aSEC, RP, CEX HPLC, DLS, CD
• Designed and executed refolding scouts to find optimal conditions for refolding
• Custom packed columns with bed volumes ranging from ml to L scale
• Performed ultrafiltration and diafiltration using TFF for up to 50 L scale
• Performed high through put protein purification
• Extensively worked on Akta Start, explorer, FPLC and Pilot
• Developed SOPs and prepared detailed report
• Trained and supervised direct report
• Trained and supervised new and old hires on the purification and characterization techniques including DLS, HPLC, TFF, custom resin packing, and Akta explorer
• Extensive client interactions to prepare statement of works, reports, and expand business opportunities in a one-to-one or team settings
• Handled multiple projects and client interactions simultaneously
• Collaborated with various teams for the completion of projects
• Directly involved in installation or training in new techniques such as DLS, Octet, and UPLC
Years: 2011 to 2016 Employer: University of Massachusetts Title: Postdoctoral Researcher Department: Responsibilities: • Investigated crosstalk between folding and aggregation of a model protein and its engineered variants
• Performed 15+ protein enrichments under minimal conditions to isotopically label protein
• Learned and performed 1D, 2D HSQC, 3D HNCO, HNCA, HNcoCA, HNcaCO, CBCAcoNH, and HNCACB experiments
• Processed NMR spectra and assigned protein under denatured condition
• Performed non-uniform sampling to reduce NMR time
• Developed a method using principal component analysis to investigate structural content of the unfolded state
• Developed skills for R, Python and MATLAB programming
• Performed protein folding experiments using CD, fluorescence measurements
• Identified that the folding information is present in the unfolded state
• Presented work in conferences and annual retreats
• Trained and supervised high school student and undergrads
Years: 2010 to 2011 Employer: Center for Cellular and Molecular Biology Title: Research Associate Department: Responsibilities: • Designed software for photo-acoustic spectroscopy (PAS) using LabVIEW
• Performed stability analysis on recombinant humanized monoclonal antibody, Bevacizumab (Avastin, Genentech/Roche) using reverse phase-HPLC (RP-HPLC)
Years: 2004 to 2010 Employer: Center for Cellular and Molecular Biology Title: Graduate Research Assistant Department: Responsibilities: • Studied misfolding and aggregation of mammalian prion protein
• Cloned, expressed and purified mammalian prion protein and its variants
• Discovered novel tertiary interactions between the N- and C-terminal regions of prion protein upon copper-binding using CD, fluorescence, SAXS, ITC, AUC, DLS, and NMR
• Identified loss of nucleation as a cause for inhibition of amyloid formation of UV-exposed prion protein using CD, fluorescence, AFM, and EM
• Authored three scientific papers and a book chapter
• Presented various professional seminars on prion diseases
• Trained and supervised high school students, undergrads and graduate students

Career Accomplishments

Awards / Recognition
HONORS AND AWARDS
• Best oral presentation award, in National Symposium on Biophysics-Biophysics in Medicine and Biology, organized by Indian Biophysical Society, India
• Young Scientist Bursary to attend 63rd Harden Conference – Protein folding and assembly in vitro and in vivo, Ambleside, UK, organized by Biochemical Society
• Senior Research Fellowship, Council of Scientific and Industrial Research (CSIR), India
• Junior Research Fellowship, CSIR, India

Additional Experience

Other Relevant Experience
ADDITIONAL INFO
• Workshop attended- Tips & Tricks: ACQUITY H-Class and QDa at Waters regional office
• Was point-of-contact with service engineers for several biophysical instruments such as ITC, CD, DLS, and time-resolved fluorometers
• Installed Endosafe Nexgen PTS reader with the remote help of application scientist

Fields of Expertise

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