Expert in Protein Biochemistry, Purification, Characterization; Gentle Immunoaffinity Chromatography
His lab studies RNA polymerases and transcription factors from bacteria (especially E. coli) and from eukaryotes including wheat, yeast, calf thymus, and human cells. He discovered the complex subunit structure of E. coli RNA polymerase and the initiation factor, sigma-70, responsible for specific RNA synthesis (transcription). He also studies the mechanism of action of the phytotoxin tagetitoxin in inhibiting RNA polymerase.
Expert teaches a 28-lecture course at the University of Wisconsin at Madison and a two week lab course at Cold Spring Harbor. He organized the Keystone Symposia on protein purification in 1986 and 1992. He has developed the use of polyethyleneimine precipitation as an important step in clarifying extracts and fractionating proteins. He has helped popularize the use of glycerol and dithiothrietol as protein stabilization agents.
As an early user of polyacrylamide gel electrophoresis (PAGE) and SDS-PAGE, he developed techniques for improving staining and quantitation of protein in gels. He dramatically improved the efficiency of eluting microgram amounts of enzymes from SDS gels, removal of SDS, and renaturation of active enzymes.
Since a sabbatical in 1983-84 with a medical diagnostic company, Expert has been very active in producing MAbs as reagents to aid in his biochemical studies. He has developed methods for rapid epitope-mapping. He has recently developed and patented a method for screening for rare MAbs ideal for immunoaffinity chromatography. These "polyol-responsive" MAb columns bind enzymes tightly but release under very gentle conditions of salt and polyol (glycerol, ethylene glycol, propylene glycol). This allows rapid immunoaffinity purification of labile enzymes.
He has considerable experience isolating enzymes from natural sources such as bacterial, yeast, wheat germ, and mammalian cell extracts. He is also very experienced at overproducing enzymes expressed from genes cloned into E. coli, solubilizing insoluble enzymes found in inclusion bodies, and refolding to yield active enzymes.
Expert has focused his career on understanding the RNA synthesis or transcription machinery. This includes RNA polymerases and transcription factors from prokaryote (mainly E. coli) and eukaryotes (wheat, yeast, calf, human). His work has involved purification, subunit characterization, immunochemical analysis, cloning and overproducing subunits, and protein-protein crosslinking.
In an area of secondary expertise, he has used heterobifunctional crosslinking reagents to determine where on RNA polymerase certain RNA polymerase-binding transcription factors are binding and thus to infer adjacency and subunit architecture.
He has used monoclonal antibodies to aid in biochemical studies of enzymes. He has developed several new ways of epitope mapping that are faster and easier than traditional protein chemical approaches.
He acted as expert witness in three biotechnology-related patent cases, in the area of protein biochemistry. He is a member of the Scientific Advisory Board for a local monoclonal antibody production company. He has consulted for several investment companies in the areas of biotechnology and technology evaluation. He has on numerous occasions consulted with biotechnology companies in the area of protein purification.
Expert may consult nationally and internationally, and is also local to the following cities: Milwaukee, Wisconsin - Madison, Wisconsin - Kenosha, Wisconsin - Racine, Wisconsin - Rockford, Illinois - Aurora, Illinois - Elgin, Illinois - Waukegan, Illinois - Arlington Heights, Illinois - Schaumburg, Illinois
|Year: 1969||Degree: Ph.D.||Subject: Biochemistry and Molecular Biology||Institution: Harvard University (with renowned genetic scientist, Professor James D. Watson)|
|Year: 1964||Degree: B.S.||Subject: Chemistry||Institution: Caltech|
|Years: 1971 to Present||Employer: Undisclosed||Title: Professor Emeritus||Department:||Responsibilities: He runs a small laboratory, teaches and participates in departmental and university activities.|
|Years: 1983 to 1984||Employer: Genetics Systems, Inc||Title: Visiting Director of Research||Department: Biochemistry||Responsibilities: Expert spent a year on a Guggenheim Fellowship in a monoclonal antibody company in Seattle WA, where he built the biochemistry division.|
|Years||Country / Region||Summary|
|Years: 1979 to 1979||Country / Region: Russia||Summary: Has lectured internationally, including six lectures in Russia as an invited guest of the Soviet Academy of Science.|
|Years: 1969 to 1971||Country / Region: Switzerland||Summary: He was a postdoctoral Fellow at the Institute for Molecular Biology in Geneva.|
|Years: 2012 to 2012||Country / Region: Germany||Summary: He was an instructor in an EMBO course on Protein Purification and Characterization in Hamburg Germany for 10 days.|
|Associations / Societies|
|AACR, Protein Society, ACS, ASBMB|
|Expert is a member of many professional societies, is currently Editor-in-Chief of the journal, Protein Expression and Purification. He is also a series editor for a series of biotechnical resource manuals published by Oxford University Press.|
|Awards / Recognition|
|Fellow of American Academy of Microbiology and AAAS, Waksman Medal, Pfizer Award, Honorary Degree from Watson School of Biological Science, Cold Spring Harbor Laboratory, Fellow of Wisconsin Academy of Sciences, Arts and Letters.|
|Publications and Patents Summary|
|He has over 240 publications and about 8 patents|
|Expert Witness Experience|
|Expert has served three times as an expert witness in the area of protein biochemistry, including expert report preparation, deposition, and testimony|
|Training / Seminars|
|He has taught Protein Purification at Cold Spring Harbor for the last 22 years and his course in Madison since 1986.|
|He founded a Biotechnology Center in 1984 and stimulated the growth of the Biotech business community in Wisconsin from 3 in 1984 to over 150 in 2009. He also has been an active angel investor, consultant in start-ups and founded an antibacterial company.|