Expert Details

Expert in Regulation of Gene Expression

Expert ID: 722070 Belgium

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Expert has more than 14 years of international experience as a researcher in the areas of endocrinology, biochemistry, molecular biology and genetics. He earned his Ph.D. from the University of Nijmegen working on the regulation of gene expression of Proopiomelanocortin (POMC) and the neuroendocrine protein, 7B2.

He has created several cell lines overproducing the mammalian pro-protein processing enzyme furin, with highly improved processing capacity for the production of recombinant proteins that require maturation at pairs of basic amino acids and have to be produced in mammalian cells. He has extensive research experience in the area of regulation of gene expression. He has cloned the promoter regions of many genes and has extensively characterized regulatory regions responsible for the regulation of the expression of genes using a large repertoire of assays including promoter truncations, reporter assays and transfection studies in a wide variety of cell lines using all current transfection procedures.

Expert has deep experience in the analysis of gene expression. He has extensive experience with Northern blot procedures (both formaldehyde and glyoxal-based methods) and has extensive experience with RNA isolation procedures. In addition, he has extensive experience with Western blot analysis to characterize protein expression using both colorimetric and chemical luminescence detection systems. He has in-dept experience in DNA amplification, cloning, site directed mutagenesis, and sequencing as well as many bioinformatics tools to characterise genes.

Expert took part in the on-site audits of the science advisory boards of the his current workplace, (Undisclosed).

Education

Year Degree Subject Institution
Year: 1991 Degree: Ph.D. Subject: Molecular Biology Institution: University of Nijmegen (The Netherlands)

Work History

Years Employer Title Department Responsibilities
Years: 1999 to Present Employer: Undisclosed Title: Undisclosed Department: Headquarters Responsibilities: Works as science advisor to (Undisclosed) for Biotechnology (VIB). The VIB is an academic institute with a budget of about USD$60 million, employing over 750 people, working in several areas of molecular biology, plant and human genetics, cell biology, immunology, cancer and neurodegeneration. The institute publishes about 300 publications per year in peer-reviewed journals, of which about 10% are in high-ranking journals (impact factor higher than 10). As a science advisor for the VIB, Expert has to stay abreast of all the science performed within the VIB and in related fields. He also has to stay abreast of the developments in the biotechnology industry and keep track of technological innovations, especially in the areas of functional genomics and bioinformatics. His job enables him to work on many scientific disciplines at a complex level, as he has to give advice on scientific matters but also on technology transfer matters.
Years: 1992 to 1998 Employer: University of Leuven (Belgium) Title: Undisclosed Department: Center of Human Genetics Responsibilities: He was a group leader on cancer biology.
Years: 1987 to 1991 Employer: University of Nijmegen (The Netherlands) Title: Undisclosed Department: Animal Physiology Responsibilities: He performed molecular biological research.

Government Experience

Years Agency Role Description
Years: 2001 to Present Agency: European Union Role: Research Manager Description: Expert assists in determining areas of cooperation between research programmes of European countries that are part of the EU.
Years: 2000 to Present Agency: University of Gent, Belgium Role: Research Integrity Officer Description: He investigates allegations of possible scientific misconduct.

International Experience

Years Country / Region Summary
Years: 2000 to 2000 Country / Region: UK, Germany, France Summary: Expert visited several top European scientific institutes (MRC, EMBL, Curie).
Years: 2000 to 2000 Country / Region: USA Summary: He visited several top U.S. scientific institutes (Salk, Scribbs)in order to determine their strategy to maintain their high level of research.
Years: 1992 to Present Country / Region: Belgium Summary: He first worked as a scientist in Belgium and later as a science advisor for the Flanders Interuniversity Institute for Biotechnology.
Years: 1987 to 1991 Country / Region: The Netherlands Summary: Expert completed his Ph.D. in The Netherlands.

Language Skills

Language Proficiency
Dutch Expert can speak, read and write Dutch.
Spanish He can speak, read and write Spanish.

Fields of Expertise

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