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Protein Chemistry & Regulation, Proteomics, Protein Mass Spectrometry, Proteases, Enzymology
Since 1990, Expert and his colleagues have analyzed proteins by electrospray or MALDI mass spectrometry to detect posttranslational modifications such as phosphorylation and fatty acylation. He has also been using it to detect genetic variants and to measure the precise…
Biochemistry and Enzymology
A primary focus of Expert E. Expert's research efforts has been the determination of the mechanism of action of enzyme-catalyzed reactions. He has applied a wide range of kinetic techniques, including steady-state and pre-steady-state studies, isotope effect studies, inhibition, inactivation,…
Food Microbiology, Toxicology, Preservation, and Spoilage; Yeasts; Clostridia; Botulinum Toxin
Expert's primary area of research is the study of botulinum toxin in the gram-positive pathogen Clostridium botulinum. Much of his work has been devoted to methods for the prevention of toxin formation in foods. He has also contributed to the…
Food Chemistry and Nutrition, Folic Acid, Vitamin Metabolite, etc.
A significant portion of Expert's research in food chemistry and nutrition has involved the development of analytical methods for various vitamins and their metabolites in foods and biological materials. This work has primarily involved the application of HPLC, with particular…
Protein Biochemistry, Purification, Characterization; Gentle Immunoaffinity Chromatography
Expert is internationally known for his contributions to the fields of biochemistry and molecular biology. He has worked in this area since 1964, generally in purifying enzymes involved in RNA synthesis, characterizing the structure and function of enzymes, and studying…
Protein Biochemistry, E Coli Mutation, Thioredoxins, Amino Acid Residues, etc.
Expert has expertise in the purification and characterization of disulfide-containing redox proteins from a variety of micro-organisms. She has used the standard techniques of both low and high pressure chromatography on ion exchange and size exclusion gels. She is also…
Biotechnology
APPLIED MICROBIOLOGY; BIOTECHNOLOGY. Expert is an authority in biotechnology, especially biotechnological principles for food, chemical, and pharmaceutical applications. He expends significant research efforts on the genetics of industrial organisms, with the ultimate goal of isolating, overproducing, and utilizing enzymes and…