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Protein Purification: Hands-on, Over 50 Projects Completed, Over 75 Proteins Purified
Expert has been actively involved in hands-on protein purification since 1985. Early work involved purification of enzymes and metal-binding proteins. From 1993 to 1997, as a Senior Scientist at a major biotech company, expert had very successfully developed methods for…
Nanotechnology, Biomaterials, Sol-gel Sciences, Optics, Biotechnology, Glass, Ceramics, Spectroscopy
Expert is a specialist of sol-gel chemistry. He developed a new non-aqueous method for sol-gel glass coating to obtain transparent films for integrated optics and photonics using polysiloxane materials. The aim was to obtain low-softening temperature glasses to permit facile…
Organic, Polymer and Surface Chemistry, Bioapplications and Separation Technologies
Expert designed and synthesized multifunctional surface chemistries for modification of glass, porous silica gels, carbon and various polymeric substrates in form of the membranes, micro particles, porous monolithic blocks, and capillaries. The following methods have been used for surface functionalzation:…
Biochemistry and Enzymology
A primary focus of Expert E. Expert's research efforts has been the determination of the mechanism of action of enzyme-catalyzed reactions. He has applied a wide range of kinetic techniques, including steady-state and pre-steady-state studies, isotope effect studies, inhibition, inactivation,…
Protein Biochemistry, E Coli Mutation, Thioredoxins, Amino Acid Residues, etc.
Expert has expertise in the purification and characterization of disulfide-containing redox proteins from a variety of micro-organisms. She has used the standard techniques of both low and high pressure chromatography on ion exchange and size exclusion gels. She is also…