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Biochemistry and Enzymology
A primary focus of Expert E. Expert's research efforts has been the determination of the mechanism of action of enzyme-catalyzed reactions. He has applied a wide range of kinetic techniques, including steady-state and pre-steady-state studies, isotope effect studies, inhibition, inactivation,…
Enzyme and Protein Technology
Expert has devoted much of his career to derivatization of surfaces, development of methods for immobilization of ligands, and characterization of affinity matrices. He pioneered development of the technique of analytical affinity chromatography and has used this technique to characterize…
Material Analysis/Spectroscopy
During the past 15 years, Expert's research has become increasingly oriented toward vibrational spectroscopy, particularly Fourier transform infrared (FT-IR) spectroscopy. In the early 1980s, he developed modern step-scan FT-IR instrumentation for photoacoustic and photothermal spectroscopy of surfaces and opaque or…
Biomolecular Research Resource
AMINO ACIDS; PEPTIDES; PROTEINS; PROTEIN PURIFICATION. Expert has devoted a considerable portion of his career to providing the capability to purify, sequence, and synthesize proteins. He has helped develop the technologies and protocols needed as well as implement the ideas…
Environmentally-Compatible Polymers
Expert is an expert in environmentally-compatible materials, including biodegradable plastics and polymers, plastic environmental effects, plastic non-hazardous solid waste management, paper coatings, and moisture barrier material. He has conducted research on the design and engineering of new biodegradable materials that…
Protein Biochemistry, E Coli Mutation, Thioredoxins, Amino Acid Residues, etc.
Expert has expertise in the purification and characterization of disulfide-containing redox proteins from a variety of micro-organisms. She has used the standard techniques of both low and high pressure chromatography on ion exchange and size exclusion gels. She is also…
Protein Biochemistry, Glycoproteins
Expert has spent much of his career involved in studies of the glycoprotein hormone family of gonadotropins. He has been involved in their protein chemistry, including purification of hormones and important natural fragments, primary structural work, and generation of antibodies.…