Expert in Analytical Chemistry, HPLC, LC/MS, GC/MS, GC/MS/MS, Data analysis, method development, qPCR, Western Blotting
Expert ID: 734253 Kentucky, USA
LC-MS/MS, GC-MSD/FID/ECD, GC-MS/MS, HPLC, Thermofisher QXactive, ABSCIEX Triple quadrupoles, Agilent Triple quadrupoles, Agilent GC/MS, Shimadzu HPLC/UHPLC systems, Analytical method development and validation for extraction, identification and quantitation of small molecules, Data analysis, Team management and training, Project management, Real time PCR, Protein purification, PCR, Cell culture, SDS-PAGE, Enzyme activity, DNA/RNA extractions, Primer design, cDNA synthesis, Reverse transcription, invitro transcription and translation, Solid phase extraction, Polymer synthesis, UV-Vis, FTIR, DSC, NMR.
|Year: 2013||Degree: Ph.D||Subject: Bioanalytical Chemistry||Institution: University of Kentucky|
|Year: 2008||Degree: MS||Subject: Analytical Chemistry||Institution: University of Nebraska-Lincoln|
|Year: 2002||Degree: M.Sc||Subject: Chemistry||Institution: Indian Institute of Technology -Delhi (IIT-D)|
|Years: 2015 to Present||Employer: Undisclosed||Title: Scientist II||Department:||Responsibilities:|
|Years: 2014 to 2015||Employer: Small Molecule Mass Spectrometry Core Laboratory, Internal Medicine, University of Kentucky||Title: Scientist I||Department:||Responsibilities:|
|Years: 2013 to 2014||Employer: Small Molecule Mass Spectrometry Core Laboratory, Internal Medicine, University of Kentucky||Title: Research Analyst||Department:||Responsibilities: Work involves providing structural analysis and quantitation of a wide range of analytes including pollutants, drugs, lipids, nucleotides, peptides and other metabolites using both LC-MS/MS and GC-MS/MS in biological fluids and tissue/environmental/chemical samples. I also work on data analysis for LC and GC MS/MS measurements with ABSciex Analyst, Analyst TOF and Agilent Masshunter/Chemstation software including peak finding, peak integration and data export. Additionally, I train students/postdocs/scientists in instrumentation and analytical techniques such as sample extractions and preparation including liquid and solid phase extraction using manual or automated systems, solvent evaporation and sample reconstitution.
|Years: 2008 to 2013||Employer: University of Kentucky.||Title: PhD candidate||Department: Dept. of Chemistry and Sanders-Brown Center on Aging||Responsibilities: My graduate work has focused on multiple aspects of oxidative DNA damage and repair within a single cohort of subjects in multiple brain regions during the progression of Alzheimer’s disease (AD). The studies have included quantification and analysis of gene expression, protein levels, and enzyme activities of multiple DNA repair proteins involved in the BER pathway including OGG1, UNG, APE1, POLB, PARP1, XRCC1, and FEN1. A second project focused on the investigation of genomic oxidative damage within the coding sequences of proteins found to be differentially regulated during the progression of AD by real-time PCR analysis.
|Years: 2008 to 2010||Employer: University of Kentucky||Title: Teaching assistant for graduate courses||Department:||Responsibilities: Biochemistry, Analytical Chemistry and Undergraduate General Chemistry, Department of Chemistry
|Years: 2004 to 2008||Employer: University of Nebraska- Lincoln||Title: Teaching assistant||Department:||Responsibilities: General and Organic Chemistry, Department of Chemistry|
|Years: 2004 to 2008||Employer: University of Nebraska-Lincoln||Title: MS||Department: Dept. of Chemistry||Responsibilities: Projects: (1) Development of new affinity monolithic chromatographic polymer supports for HPLC columns, (2) Development of new techniques to make affinity monolithic polymer microcolumns for HPLC and (3) Characterization of drug-binding between drugs such as lidocaine and verapamil with the protein, human serum albumin using UPLC/RPLC methods.|
|Years: 2004 to 2004||Employer: University of Nebraska-Lincoln||Title: Research Assistant||Department: Dept. of Chemistry||Responsibilities:|
|Years: 2003 to 2004||Employer: Centre for Polymer Science and Engg.||Title: Research Associate||Department:||Responsibilities: Projects: Analysis of resin from the Impregnated Filter Paper and development of water based phenolic and acrylic resin.|
|Years: 2002 to 2003||Employer: IIT||Title: Research Associate||Department: Dept. of Civil Engg.||Responsibilities: Project: Chemical/biochemical/bacteriological analysis of river & drain water resources in Delhi.|
|Years: 2002 to 2002||Employer: Centre for Biomedical Engg., IIT||Title: Project Associate||Department:||Responsibilities: Project: Effect of excess DMSO and styrene on the DNA of human cells and on RISUG (an experimental reversible male contraceptive in its 3rd stage of clinical trial).
|Years: 2001 to 2002||Employer: IIT||Title: M.Sc. Project||Department: Dept. of Chemistry||Responsibilities: Projects: (1) Development of a purification method for effective & economical purification of the enzyme porcine pancreatic alpha-amylase using reversibly soluble insoluble (SIS) polymers and (2) Molecular Imprinting of Eudragit with tartaric acid.
|Awards / Recognition|
|1. Research Challenge Trust Fund (RCTF) Fellowship, UK
2. Graduate School Travel Fellowship for Graduate Students, UK (2 years)
3. Fast Track Research Award, Department of Chemistry, UK
4. Sigma Xi Graduate Outstanding Poster award at the Nebraska Science Fair
5. Qualified Council of Scientific and Industrial Research (CSIR)- National Eligibility Test (All India open exam)
6. Qualified Graduate Aptitude Test for Engg. (GATE), All India open exam
|Publications and Patents Summary|
Research Presentations: 14
|English||Native or bilingual proficiency|
|Hindi||Native or bilingual proficiency|
|Malayalam||Native or bilingual proficiency|
|Bengali||Professional working proficiency|