Expert Details

Expert in Analytical Chemistry, HPLC, LC/MS, GC/MS, GC/MS/MS, Data analysis, method development, qPCR, Western Blotting

Expert ID: 734253 Kentucky, USA

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Areas of expertise:
LC-MS/MS, GC-MSD/FID/ECD, GC-MS/MS, HPLC, Thermofisher QXactive, ABSCIEX Triple quadrupoles, Agilent Triple quadrupoles, Agilent GC/MS, Shimadzu HPLC/UHPLC systems, Analytical method development and validation for extraction, identification and quantitation of small molecules, Data analysis, Team management and training, Project management, Real time PCR, Protein purification, PCR, Cell culture, SDS-PAGE, Enzyme activity, DNA/RNA extractions, Primer design, cDNA synthesis, Reverse transcription, invitro transcription and translation, Solid phase extraction, Polymer synthesis, UV-Vis, FTIR, DSC, NMR.

Education

Year Degree Subject Institution
Year: 2013 Degree: Ph.D Subject: Bioanalytical Chemistry Institution: University of Kentucky
Year: 2008 Degree: MS Subject: Analytical Chemistry Institution: University of Nebraska-Lincoln
Year: 2002 Degree: M.Sc Subject: Chemistry Institution: Indian Institute of Technology -Delhi (IIT-D)

Work History

Years Employer Title Department Responsibilities
Years: 2015 to Present Employer: Undisclosed Title: Scientist II Department: Responsibilities:
Years: 2014 to 2015 Employer: Small Molecule Mass Spectrometry Core Laboratory, Internal Medicine, University of Kentucky Title: Scientist I Department: Responsibilities:
Years: 2013 to 2014 Employer: Small Molecule Mass Spectrometry Core Laboratory, Internal Medicine, University of Kentucky Title: Research Analyst Department: Responsibilities: Work involves providing structural analysis and quantitation of a wide range of analytes including pollutants, drugs, lipids, nucleotides, peptides and other metabolites using both LC-MS/MS and GC-MS/MS in biological fluids and tissue/environmental/chemical samples. I also work on data analysis for LC and GC MS/MS measurements with ABSciex Analyst, Analyst TOF and Agilent Masshunter/Chemstation software including peak finding, peak integration and data export. Additionally, I train students/postdocs/scientists in instrumentation and analytical techniques such as sample extractions and preparation including liquid and solid phase extraction using manual or automated systems, solvent evaporation and sample reconstitution.
Years: 2008 to 2013 Employer: University of Kentucky. Title: PhD candidate Department: Dept. of Chemistry and Sanders-Brown Center on Aging Responsibilities: My graduate work has focused on multiple aspects of oxidative DNA damage and repair within a single cohort of subjects in multiple brain regions during the progression of Alzheimer’s disease (AD). The studies have included quantification and analysis of gene expression, protein levels, and enzyme activities of multiple DNA repair proteins involved in the BER pathway including OGG1, UNG, APE1, POLB, PARP1, XRCC1, and FEN1. A second project focused on the investigation of genomic oxidative damage within the coding sequences of proteins found to be differentially regulated during the progression of AD by real-time PCR analysis.
Years: 2008 to 2010 Employer: University of Kentucky Title: Teaching assistant for graduate courses Department: Responsibilities: Biochemistry, Analytical Chemistry and Undergraduate General Chemistry, Department of Chemistry
Years: 2004 to 2008 Employer: University of Nebraska- Lincoln Title: Teaching assistant Department: Responsibilities: General and Organic Chemistry, Department of Chemistry
Years: 2004 to 2008 Employer: University of Nebraska-Lincoln Title: MS Department: Dept. of Chemistry Responsibilities: Projects: (1) Development of new affinity monolithic chromatographic polymer supports for HPLC columns, (2) Development of new techniques to make affinity monolithic polymer microcolumns for HPLC and (3) Characterization of drug-binding between drugs such as lidocaine and verapamil with the protein, human serum albumin using UPLC/RPLC methods.
Years: 2004 to 2004 Employer: University of Nebraska-Lincoln Title: Research Assistant Department: Dept. of Chemistry Responsibilities:
Years: 2003 to 2004 Employer: Centre for Polymer Science and Engg. Title: Research Associate Department: Responsibilities: Projects: Analysis of resin from the Impregnated Filter Paper and development of water based phenolic and acrylic resin.
Years: 2002 to 2003 Employer: IIT Title: Research Associate Department: Dept. of Civil Engg. Responsibilities: Project: Chemical/biochemical/bacteriological analysis of river & drain water resources in Delhi.
Years: 2002 to 2002 Employer: Centre for Biomedical Engg., IIT Title: Project Associate Department: Responsibilities: Project: Effect of excess DMSO and styrene on the DNA of human cells and on RISUG (an experimental reversible male contraceptive in its 3rd stage of clinical trial).
Years: 2001 to 2002 Employer: IIT Title: M.Sc. Project Department: Dept. of Chemistry Responsibilities: Projects: (1) Development of a purification method for effective & economical purification of the enzyme porcine pancreatic alpha-amylase using reversibly soluble insoluble (SIS) polymers and (2) Molecular Imprinting of Eudragit with tartaric acid.

Career Accomplishments

Awards / Recognition
1. Research Challenge Trust Fund (RCTF) Fellowship, UK
2. Graduate School Travel Fellowship for Graduate Students, UK (2 years)
3. Fast Track Research Award, Department of Chemistry, UK
4. Sigma Xi Graduate Outstanding Poster award at the Nebraska Science Fair
5. Qualified Council of Scientific and Industrial Research (CSIR)- National Eligibility Test (All India open exam)
6. Qualified Graduate Aptitude Test for Engg. (GATE), All India open exam
Publications and Patents Summary
Publications: 14
Research Presentations: 14

Language Skills

Language Proficiency
English Native or bilingual proficiency
Hindi Native or bilingual proficiency
Malayalam Native or bilingual proficiency
Bengali Professional working proficiency

Fields of Expertise

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