Expert Details

Expert in Assay Development/Molecular Biology

Expert ID: 723399 Indiana, USA

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The expert's private consulting and research company was established to provide innovative solutions to technical problems for researchers in academia and industry. Its charter is to develop strategies and protocols that streamline scientific research in the most time/cost effective ways.

Research Scientist/Consultant Services:

-Developed nucleic acid assay/detection system—compact, portable (for in-field use)—for identification of infectious diseases or genomic dispositions.

-Reviewed/reported American Red Cross Biomedical Services SOP’s in accordance with the Consent Decree (on 04/15/2003) between the American Red Cross (ARC) and the Food and Drug Administration—administered by Quintiles Consulting, Inc.

-Validated genotyping assays. Developed real-time PCR diagnostic assays for EraGen Biosciences, Inc.

-Formulated and validated novel proprietary/system for sequencing of genomic DNA for Genome Technologies, Inc.

-Examined and developed critical QC assays for PowderJect Vaccines, Inc.

-Developed/standardized assays for evaluating Pfu DNA polymerase for Promega Corporation.

-Reformulated buffer/enzyme system for Access RT- PCR System for Promega Corporation.

-Developed technology for automated sample preparation (for use in nucleic acid analysis). Sold rights to BioRad.

-Produced fluorescent/capture oligonucleotide probes and target sequences for automated instrument for Spherotech, Inc.

-Assisted in the development of rapid, colorimetric assay for detection of Chlamydia for Pacific Biotech, Inc.

-Assisted in instrument integration of nucleic acid probe assays for HIV1 and Hepatitis B for Chiron Corp.

Expert’s Scientific and Technical Skills:

DNA/RNA isolation
Sample processing/preparation--tissue cultures, bacterial cultures, animal tissues and body fluids including human;
Nucleic acid amplification-- PCR, RT-PCR, Real-time PCR, QPCR, 3SR/TAS (NASBA), LCR, etc;
Prokaryotic/eukaryotic expression;
DNA sequencing--radioisotopic, automated, etc;
Genetic cloning;
Mutation detection;
Tissue culture maintenance;
Immunoassay--ELISA/EIA, radioisotopic, agglutination, etc;
Purification methodologies--gel filtration, FPLC, HPLC, affinity chromotagraphy, extractions, etc;
Nucleic acid analysis--western blot, southern blot, northern blot;
Detection technologies--chemiluminescent, fluorescent, colorimetric;
Organic chemistries--nucleic acid, enzyme, ligand, solid-phase conjugations;

COMPUTER SKILLS

-Spreadsheet--Lotus 123/macros, Excel, Quatro Pro;
-Word processing--MSWord, WordPerfect, others;
-Nucleic Acid Analysis--GCG programs, Primer Designer, Oligo, others;
-Sequencing--ABI Software (Navigator w/, Factura w/, AutoAssembler)

Expert may consult nationally and internationally, and is also local to the following cities: Chicago, Illinois - Rockford, Illinois - Aurora, Illinois - Naperville, Illinois - Joliet, Illinois - Elgin, Illinois - South Bend, Indiana - Gary, Indiana - Milwaukee, Wisconsin - Kenosha, Wisconsin

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Education

Year Degree Subject Institution
Year: 1983 Degree: BS Subject: Microbiology Institution: University of Notre Dame

Work History

Years Employer Title Department Responsibilities
Years: 1997 to Present Employer: Undisclosed Title: Research Scientist/Consultant Department: Undisclosed Responsibilities: This private consulting and research company was established to provide innovative solutions to technical problems for researchers in academia and industry. Its charter is to develop strategies and protocols that streamline scientific research in the most time/cost effective ways.

Research Scientist/Consultant

-Developed nucleic acid assay/detection system—compact, portable (for in-field use)—for identification of infectious diseases or genomic dispositions.

-Reviewed/reported American Red Cross Biomedical Services SOP’s in accordance with the Consent Decree (on 04/15/2003) between the American Red Cross (ARC) and the Food and Drug Administration—administered by Quintiles Consulting, Inc.

-Validated genotyping assays. Developed real-time PCR diagnostic assays for EraGen Biosciences, Inc.

-Formulated and validated novel proprietary/system for sequencing of genomic DNA for Genome Technologies, Inc.

-Examined and developed critical QC assays for PowderJect Vaccines, Inc.

-Developed/standardized assays for evaluating Pfu DNA polymerase for Promega Corporation. (See Promega Notes 68)

-Reformulated buffer/enzyme system for Access RT- PCR System for Promega Corporation.
Years: 1993 to 1997 Employer: Undisclosed Title: Research Laboratory Manager Department: Undisclosed Responsibilities: Undisclosed was established at this educational institution through a contract with the National Institute for Health. This laboratory was chartered with the maintenance of various viral strains and the development/assessment of techniques within the ACTG.

Research Laboratory Manager

-Instituted/standardized assays for detection of drug-resistant mutants of HIV1. Developed sample preparation, RT-PCR, PCR and sequencing protocols in alliance with ABI, Stanford University, etc.

-Instituted/standardized assays for detection of drug-resistant mutants of CMV. Cloned and expressed CMV DNA polymerase gene in eukaryotic vector.

-Developed assay for detection of Borrelia burgdorferi using 3SR and PCR with fluorescence polarization. (Baxter Grant)
Years: 1991 to 1994 Employer: Self Title: Research Scientist/Consultant Department: Undisclosed Responsibilities: -Developed technology for automated sample preparation (for use in nucleic acid analysis). Sold rights to (Undisclosed).

-Produced fluorescent/capture oligonucleotide probes and target sequences for automated instrument for Spherotech, Inc.

-Assisted in the development of rapid, colorimetric assay for detection of Chlamydia for Pacific Biotech, Inc.

-Assisted in instrument integration of nucleic acid probe assays for HIV1 and Hepatitis B for Chiron Corp.
Years: 1988 to 1991 Employer: Undisclosed Title: Research Scientist, Project Leader Department: Undisclosed Responsibilities: Research Scientist, Project Leader

-Performed evaluation/confirmation of HIV1, HIV2, HTLV1, Hepatitis B, and Lyme disease positive samples.

-Developed homogeneous nucleic acid probe assay using fluorescence polarization.
-Implemented protocols for use of 3SR, TAS, and PCR amplification of nucleic acid in the detection of infectious agents in blood.

-Evaluated numerous nucleic acid detection technologies--HPCE/laser detection, chemiluminescence, etc.

-Directed the development of a fluorescence-based alanine aminotransferase (or SGPT) assay.

-Wrote quality control and quality assurance documents for SGPT assay production. Wrote 510 document for FDA approval of SGPT assay.
Years: 1983 to 1988 Employer: Undisclosed Title: Research Associate, Project Leader Department: Undisclosed Responsibilities: Research Associate, Project Leader

-Develop agglutination assay for rapid detection of HTLV1.
-Evaluated/developed EIA assay for detection Rotavirus. Organized clinical study.
-Researched/evaluated new and altered strains of Bacillus thuringiensis for production of bioinsecticides.
-Developed/evaluated chemistries for detection of Herpes simplex 1.

Fields of Expertise

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