Expert Details

Expert in Biochemistry and Enzymology

Expert ID: 720680 Ohio, USA

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A primary focus of Expert E. Expert's research efforts has been the determination of the mechanism of action of enzyme-catalyzed reactions. He has applied a wide range of kinetic techniques, including steady-state and pre-steady-state studies, isotope effect studies, inhibition, inactivation, pH dependent, and temperature dependent studies, to a variety of bacterial, yeast, and mammalian enzyme systems.

The study of enzyme structure and enzyme mechanisms has remained the primary objective of Expert's research efforts. A wide range of experimental approaches have been used in his enzymological studies, ranging from techniques that are quite biological in nature (such as site-directed mutagenesis), to chemical techniques (such as chemical and active-site-directed modification studies), to physical and spectroscopic techniques (such as UV/visible, fluorescence, NMR, EPR, and X-ray crystallography.

The role of metal ions in biological systems and, in particular, the function of divalent cations in enzyme-catalyzed reactions remains an important area of interest. Studies in Expert's laboratory have ranged from the structural characterization of metal-amino acid and metal-peptide complexes, to metal binding plant lectins, to metal-activated and metalloenzymes. His work in this area has also included a study of the interaction of platinum anti-cancer drugs with nucleotides, and the formation of aluminum complexes of biological ligands.

Expert's research group has developed an extensive expertise in the purification of peptides and proteins. The experimental approaches that he has used range from classical techniques (such as gel filtration and ion exchange chromatography), to affinity techniques (such as hydrophobic chromatography, dye ligand chromatography, and specific ligand immobilization), to newer HPLC purification techniques.

Expert's research group is exploring methods for the selective introduction of unnatural amino acids into enzymes with the goal of altering their substrate specificity and modifying their catalytic efficiency.

X-ray diffraction studies are a powerful tool to determine macromolecular structures at atomic resolution. The Ohio Crystallography Consortium at the University of Toledo provides both instrumental capabilities and experimental expertise to solve protein structures. Expert's llaboratory is actively investigating the structures of a range of enzymes.

Expert may consult nationally and internationally, and is also local to the following cities: Detroit, Michigan - Warren, Michigan - Flint, Michigan - Sterling Heights, Michigan - Lansing, Michigan - Ann Arbor, Michigan - Livonia, Michigan - Cleveland, Ohio - Toledo, Ohio - Fort Wayne, Indiana

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Education

Year Degree Subject Institution
Year: 1976 Degree: PhD Subject: Biochemistry Institution: Pennsylvania State University
Year: 1973 Degree: MS Subject: Chemistry Institution: Pennsylvania State University
Year: 1967 Degree: BS Subject: Chemistry Institution: Fordham University

Work History

Years Employer Title Department Responsibilities
Years: 2000 to Present Employer: Undisclosed Title: Professor Department: Department of Chemistry Responsibilities:
Years: 1989 to 2000 Employer: University of Akron Title: Professor Department: Department of Chemistry Responsibilities:
Years: 1984 to 1989 Employer: University of Akron Title: Associate Professor Department: Department of Chemistry Responsibilities:
Years: 1979 to 1984 Employer: Southern Illinois University Title: Assistant Professor Department: Department of Chemistry Responsibilities:
Years: 1977 to 1979 Employer: University of Wisconsin Title: NIH Postdoctoral Fellow Department: Department of Biochemistry Responsibilities:

Career Accomplishments

Associations / Societies
Expert is a member of the American Chemical Society (ACS), the Protein Society, and the American Society of Biochemistry and Molecular Biology (ASBMB).
Publications and Patents Summary
He has 57 publications and two patents in biochemistry and enzymology.

Fields of Expertise

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