Expert in Blood-Biomaterial Interactions, Platelets, Platelet-Rich Plasma, Surface Analysis, Flow Cytometry, Neutron Reflectometry, and Lipid Biophysics
Expert ID: 733508 Germany
His expertise includes the design of QCM instruments, as well as their applications in both nanoparticles and liposomes.
Expert has more than five years hands-on and leadership experience in the analysis of blood-biomaterial interactions, platelet activation at biomaterial surfaces, and platelet-leukocyte interactions. His expertise also includes:
• Design of flow cytometry assays for analyzing platelet activation
• Blood handling (platelet isolation, red blood cell isolation, etc.)
• Blood cell analysis
He is an expert in liposome preparation and characterization. This expertise includes Phoshatidilserine, interactions with proteins, coagulation, and lipid asymmetry.
|Year: 2000||Degree: Ph.D.||Subject: Mathematics and Natural Sciences (Biophysics)||Institution: University of Groningen, the Netherlands|
|Year: 1996||Degree: B.Sc.||Subject: Biochemistry||Institution: McMaster University, Onrario, Canada|
|Years: 2013 to Present||Employer: Undisclosed||Title: Groupleader||Department: Institute for Functional Interfaces||Responsibilities: Established and led an independent research laboratory studying platelets, platelet activation, and their role in material hemocompatibility of vascular implants.
Mentored Doctoral, Master, and undergraduate students.
Organized conferences on blood-biomaterial interactions.
Research group activities included the design of whole-blood, platelet-rich plasma, and purified platelet activation assays and microfluidic platelet secretion assays.
|Years: 2006 to 2013||Employer: CIC biomaGUNE||Title: Groupleader||Department:||Responsibilities: Established and led an independent research group in the area of biological surfaces and interfaces with the focus on lipid assemblies and platelet-biomaterial interactions
Organized institute-wide surface preparation and analysis facilities consisting of magnetron sputtering, X-ray photoelectron spectroscopy, and atomic force microscopy instruments
Mentored Doctoral, Master, undergraduate students, and postdoctoral researchers; supervised technical assistants and facility managers.
Organized educational workshops, taught practical courses on lipid handling and liposome preparation, atomic force microscopy, UHV surface analysis, quartz crystal microbalance;
Group research activities included
Developing surface-acoustic based methods for measuring sizes of surface-adsorbed particles, such as liposomes, in particular, their deformation upon adsorption
Unraveling hydrodynamic mechanisms in surface-acoustic sensing
Developing lectin-based platelet activation assay and demonstrating agonist-specific activation effects
Establishing platelet activation selectivity at biomaterial surfaces and therefore developing the concept of “biomaterial as a platelet agonist."
|Years: 2005 to 2006||Employer: Clausthal University of Technology||Title: Postdoc||Department: Physical Chemistry||Responsibilities: Shear-acoustic detection in laterally heterogeneous, ultrathin biomolecular films.
The design of a combined AFM-QCM instrument.
|Years: 2002 to 2004||Employer: University of Houston||Title: Postdoc||Department: Chemical Engineering||Responsibilities: In situ analysis of 3D protein crystal growth with high-resolution atomic force microscopy.
The work involved soluble proteins (insulin) and transmembrane proteins (bacteriorhodopsin crystallized in cubo).
Analysis of microscopy images of periodic (crystalline) and non-periodic (single particles) specimens (GRIP and EMAN software packages).
|Years: 2000 to 2002||Employer: Swiss Federal Institute of Technology Zurich||Title: Postdoc||Department: Materials||Responsibilities: Lipid-surface interactions, nanopatterning of oxide surfaces and their characterization with ultra-high vacuum and "wet" surface-analytical methodologies such as X-ray photoelectron spectroscopy, atomic force microscopy in liquid, and quartz crystal microbalance.
Co-supervision of Doctoral and Master students.
|Years: 1996 to 2000||Employer: University of Groningen||Title: PhD student||Department: Biophysical Chemistry||Responsibilities: Ultrahigh Resolution Atomic Force Microscopy of Biological Macromolecules and Their Assemblies in liquid.
In situ analysis of lipid and membrane-bound protein crystallization dynamics with AFM.
|Years||Country / Region||Summary|
|Years: 1992 to 1994||Country / Region: Canada||Summary: Undergraduate student|
|Years: 1994 to 1995||Country / Region: UK||Summary: Visiting student|
|Years: 1995 to 1996||Country / Region: Canada||Summary: Undergraduate student|
|Years: 1996 to 2000||Country / Region: the Netherlands||Summary: Doctoral (PhD) student|
|Years: 2000 to 2002||Country / Region: Switzerland||Summary: Postdoc|
|Years: 2002 to 2004||Country / Region: USA||Summary: Postdoc|
|Years: 2005 to 2006||Country / Region: Germany||Summary: Postdoc|
|Years: 2006 to 2013||Country / Region: Spain||Summary: Groupleader and Professor|
|Years: 2013 to 2016||Country / Region: Germany||Summary: Groupleader|
|Years: 2009 to 2015||Country / Region: France||Summary: Short Visits, neutron reflectometry experiments|
|Associations / Societies|
|American Physical Society
|Affiliate Professor, Department of Bioengineering, University of Washington – Seattle, WA, USA.
Editorial Board Member (Biological Physics) of Scientific Reports
Member of Subcommittee 8 ‘Biology’ of the Institut Laue-Langevin (ILL), Grenoble, France. Review of neutron beamtime proposals
Professor (part-time) a the Faculty of Engineering, the University of the Basque Country, Bilbao, Spain
Professor (part-time) at the Department of Biochemistry and Molecular Biology, the University of the Basque Country, Leioa, Spain
|Awards / Recognition|
|Alexander von Humboldt Renewed Research Stay Fellowship (November 2012).
Alexander von Humboldt Research Fellowship (October 2005 – September 2006).
2001 Paper of the Year award from the Journal of Structural Biology.
Ubbo Emmius Doctoral Fellowship at the University of Groningen (the Netherlands, 1996 – 2000).
Poster Honorable Mention award at the Keck/GCC 2002 Bioinformatics Symposium (October 2002, Rice University, Houston, TX, USA).
Conference Logo Contest winner for the Keck/GCC 2003 Annual Research Conference (October 2003, Clear Lake, Houston, TX, USA).
|Publications and Patents Summary|
|Over 50 publications in international peer-reviewed journal in the areas of lipid biophysics, biological surface analysis, and biomedicine (focus on blood-biomaterial interactions).|
|Training / Seminars|
|Numerous lectures and practical courses in the areas of surface analysis, atomic force microscopy, quartz crystal microabalance, lipid biophysics, liposome preparation and characterization, etc.|
|Selected surface analysis instruments, water purification for laboratories, flow cytometers, hematocounters, UV-Ozone cleaners, sputter coaters, fluorescence and and atomic force microscopes for purchase,|
|Other Relevant Experience|
|Design and implementation of flow-cytometric platelet activation assays (whole blood, platelet-rich plasma, purified platelets).|
|German||Reading: B1, Speaking: A2; Writing: basic|
Fields of Expertise
confocal microscopy, neutron reflectivity, quartz crystal device, transmission electron microscopy, atomic force microscope, secondary-ion mass spectroscopy, X-ray photoelectron spectroscopy, chemical surface analysis, blood-wetted biomaterial, blood wetting, stent, fluorescence microscopy, enzyme-linked immunosorbent assay, flow cytometry procedure, flow cytometry, blood analysis, blood-compatible material, blood platelet, blood coagulation, cytometry, blood, bioimplantable material, biocompatible material, dynamic light scattering spectroscopy, liposome, cell membrane biophysics, lecithin, phospholipid, research writing, proposal writing, technical writing