Expert Details

Expert in Blood-Biomaterial Interactions, Platelets, Platelet-Rich Plasma, Surface Analysis, Flow Cytometry, Neutron Reflectometry, and Lipid Biophysics

Expert ID: 733508 Germany

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Expert has over 20 years of experience in surface analysis in vacuum and in liquids. His expertise includes techniques such as ToF-SIMS, XPS, AFM, QCM and QCM-D, and neutron reflectometry. He is also very familiar with biological and biomedical applications of ToF-SIMS. His expertise includes high-resolution biological AFM in liquids. He is also highly well-versed in the reparation of thin oxide films by sputtering and their analysis, including interactions with lipids, blood, platelets.

His expertise includes the design of QCM instruments, as well as their applications in both nanoparticles and liposomes.

Expert has more than five years hands-on and leadership experience in the analysis of blood-biomaterial interactions, platelet activation at biomaterial surfaces, and platelet-leukocyte interactions. His expertise also includes:

• Hemocompatibility
• ISO10993-4
• Design of flow cytometry assays for analyzing platelet activation
• Blood handling (platelet isolation, red blood cell isolation, etc.)
• Blood cell analysis

He is an expert in liposome preparation and characterization. This expertise includes Phoshatidilserine, interactions with proteins, coagulation, and lipid asymmetry.

Education

Year Degree Subject Institution
Year: 2000 Degree: Ph.D. Subject: Mathematics and Natural Sciences (Biophysics) Institution: University of Groningen, the Netherlands
Year: 1996 Degree: B.Sc. Subject: Biochemistry Institution: McMaster University, Onrario, Canada

Work History

Years Employer Title Department Responsibilities
Years: 2013 to Present Employer: Undisclosed Title: Groupleader Department: Institute for Functional Interfaces Responsibilities: Established and led an independent research laboratory studying platelets, platelet activation, and their role in material hemocompatibility of vascular implants.

Mentored Doctoral, Master, and undergraduate students.

Organized conferences on blood-biomaterial interactions.

Research group activities included the design of whole-blood, platelet-rich plasma, and purified platelet activation assays and microfluidic platelet secretion assays.
Years: 2006 to 2013 Employer: CIC biomaGUNE Title: Groupleader Department: Responsibilities: Established and led an independent research group in the area of biological surfaces and interfaces with the focus on lipid assemblies and platelet-biomaterial interactions

Organized institute-wide surface preparation and analysis facilities consisting of magnetron sputtering, X-ray photoelectron spectroscopy, and atomic force microscopy instruments

Mentored Doctoral, Master, undergraduate students, and postdoctoral researchers; supervised technical assistants and facility managers.

Organized educational workshops, taught practical courses on lipid handling and liposome preparation, atomic force microscopy, UHV surface analysis, quartz crystal microbalance;

Group research activities included

Developing surface-acoustic based methods for measuring sizes of surface-adsorbed particles, such as liposomes, in particular, their deformation upon adsorption

Unraveling hydrodynamic mechanisms in surface-acoustic sensing
Developing lectin-based platelet activation assay and demonstrating agonist-specific activation effects

Establishing platelet activation selectivity at biomaterial surfaces and therefore developing the concept of “biomaterial as a platelet agonist."
Years: 2005 to 2006 Employer: Clausthal University of Technology Title: Postdoc Department: Physical Chemistry Responsibilities: Shear-acoustic detection in laterally heterogeneous, ultrathin biomolecular films.

The design of a combined AFM-QCM instrument.
Years: 2002 to 2004 Employer: University of Houston Title: Postdoc Department: Chemical Engineering Responsibilities: In situ analysis of 3D protein crystal growth with high-resolution atomic force microscopy.

The work involved soluble proteins (insulin) and transmembrane proteins (bacteriorhodopsin crystallized in cubo).

Analysis of microscopy images of periodic (crystalline) and non-periodic (single particles) specimens (GRIP and EMAN software packages).
Years: 2000 to 2002 Employer: Swiss Federal Institute of Technology Zurich Title: Postdoc Department: Materials Responsibilities: Lipid-surface interactions, nanopatterning of oxide surfaces and their characterization with ultra-high vacuum and "wet" surface-analytical methodologies such as X-ray photoelectron spectroscopy, atomic force microscopy in liquid, and quartz crystal microbalance.

Co-supervision of Doctoral and Master students.
Years: 1996 to 2000 Employer: University of Groningen Title: PhD student Department: Biophysical Chemistry Responsibilities: Ultrahigh Resolution Atomic Force Microscopy of Biological Macromolecules and Their Assemblies in liquid.

In situ analysis of lipid and membrane-bound protein crystallization dynamics with AFM.

International Experience

Years Country / Region Summary
Years: 1992 to 1994 Country / Region: Canada Summary: Undergraduate student
Years: 1994 to 1995 Country / Region: UK Summary: Visiting student
Years: 1995 to 1996 Country / Region: Canada Summary: Undergraduate student
Years: 1996 to 2000 Country / Region: the Netherlands Summary: Doctoral (PhD) student
Years: 2000 to 2002 Country / Region: Switzerland Summary: Postdoc
Years: 2002 to 2004 Country / Region: USA Summary: Postdoc
Years: 2005 to 2006 Country / Region: Germany Summary: Postdoc
Years: 2006 to 2013 Country / Region: Spain Summary: Groupleader and Professor
Years: 2013 to 2016 Country / Region: Germany Summary: Groupleader
Years: 2009 to 2015 Country / Region: France Summary: Short Visits, neutron reflectometry experiments

Career Accomplishments

Associations / Societies
American Physical Society
Biophysical Society
Professional Appointments
Affiliate Professor, Department of Bioengineering, University of Washington – Seattle, WA, USA.

Editorial Board Member (Biological Physics) of Scientific Reports

Member of Subcommittee 8 ‘Biology’ of the Institut Laue-Langevin (ILL), Grenoble, France. Review of neutron beamtime proposals

Professor (part-time) a the Faculty of Engineering, the University of the Basque Country, Bilbao, Spain
Professor (part-time) at the Department of Biochemistry and Molecular Biology, the University of the Basque Country, Leioa, Spain
Awards / Recognition
Alexander von Humboldt Renewed Research Stay Fellowship (November 2012).

Alexander von Humboldt Research Fellowship (October 2005 – September 2006).

2001 Paper of the Year award from the Journal of Structural Biology.

Ubbo Emmius Doctoral Fellowship at the University of Groningen (the Netherlands, 1996 – 2000).

Poster Honorable Mention award at the Keck/GCC 2002 Bioinformatics Symposium (October 2002, Rice University, Houston, TX, USA).
Conference Logo Contest winner for the Keck/GCC 2003 Annual Research Conference (October 2003, Clear Lake, Houston, TX, USA).
Publications and Patents Summary
Over 50 publications in international peer-reviewed journal in the areas of lipid biophysics, biological surface analysis, and biomedicine (focus on blood-biomaterial interactions).

Additional Experience

Training / Seminars
Numerous lectures and practical courses in the areas of surface analysis, atomic force microscopy, quartz crystal microabalance, lipid biophysics, liposome preparation and characterization, etc.
Vendor Selection
Selected surface analysis instruments, water purification for laboratories, flow cytometers, hematocounters, UV-Ozone cleaners, sputter coaters, fluorescence and and atomic force microscopes for purchase,
Other Relevant Experience
Design and implementation of flow-cytometric platelet activation assays (whole blood, platelet-rich plasma, purified platelets).

Language Skills

Language Proficiency
English Fluent (Near-native)
Russian Fluent (Native)
German Reading: B1, Speaking: A2; Writing: basic
Spanish Speaking: basic

Fields of Expertise

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