Request Expert

Has worked in the area of plant tissue culture, plant genetic engineering and genetic transformation using particle bombardment, with crops such as sorghum and pearl millet. Also, constructed cDNA libraries. He is currently genomics and bioinformatics of Pierce's disease in grapes.

Has worked with development of PCR based chromosomal markers for detection and differentiation of several bacterial species such as Bacillus, Xylella fastidiosa and Huanglongbing (Citrus greening) bacterium. Is an expert in mining SSR and Tandem repeats markers from genomic data for developing strain differentiation and diversity analysis studies.

Has good programming knowledge of PHP, PERL, HTML and MYSQL. Development of relational databases. Took part in developing two software packages SCPrimer and Nway comparison and developed online databases. Extensive experience in whole genome alignment programs such as MUMMER, MVA, MAUVE, and tools such as GenomeViz, RepeatMasker and other related software.
Experienced in handling large experimental and bioinformatics data sets and mining of data from various public repositories.
DNA sequencing, processing and contig generation using CAP3, LUCY and other related software. Experience in designing and test primers in all popular formats.
Knowledge of using a wide variety of computer software for phylogenomics (MEGA and others) and bioinformatics tools.

Experienced in using different gene isolation techniques like RT-PCR, RACE, and magnetic bead -clone capture. Construction and maintenance of cDNA libraries. Real-time quantitative RT-PCR
Conducted (using Cephid and ABI-7900 HTP system, Agilent analysis) for functional characterization of the isolated P450 monooxygenase genes. Cloning of the cDNAs into Aspergillus niger expression vectors. Routine handling of protoplast preparation and transformation. Cloning into E.coli vectors and conducting basic expression studies.

Established in vitro plant regeneration systems from a variety of explants like immature inflorescence, immature embryo, shoot tip and shoot meristems of sorghum and Pearl Millet.

Worked on genetic transformation of sorghum using Bt gene constructs, assay of transformants for studying integration, expression and inheritance of transgenes.

Designed oligos for custom microarray to study global gene expression in Vitis spps, Xylella fastidiosa, Bacillus anthracis and Phanerochaete chrysosporium. Experience in handling, printing, hybridization and scanning stages of microarrays.

Extensive knowledge in using microarray data analysis software’s such as MEV, SAM and gene spring 4.0 and data interpretation. Experience in data integration with Gene ontologies and use of software’s like GenMAPP2 ermineJ and Pathway explorer.

Cloning of the cDNAs into Aspergillus niger expression vectors. Routine handling of protoplast preparation and transformation.

Cloning into E.coli vectors and conducting basic expression studies.

Has extensive experience in handling whole genome sequence for annotation, detection of sequence variation and development of relational databases.

Has done functional genomic analysis of the Xylella fastidiosa and Vitis spps. interaction using microarray and bioinformatics tools. This includes, generation of transcription profiles for the plant-pathogen interaction and identification and quantification of the micro and macro genomic variability among the different Xylella isolates and correlate it with the pathogen virulence.

for the biothreat agents Bacillus anthracis and Francisella tularensis, using MUMMER and such other bioinformatics software’s, a whole genome search for SNPs, INDELs and similar other variable regions was conducted for the above two biothreat agents. Multiplex PCR based assay has been standardized for B. anthracis and screening of these primers across other Bacillus spp has been completed. Similarly, genome-wide search was conducted for STRs (short tandem repeats) in the above two genomes.

Is an expert in designing oligos primers across phyla and for several molecular biology experiments such as gene cloning, species detection and strain differentiation. has developed software tools for designing PCR primers.

He established in vitro plant regeneration systems from a variety of explants like immature inflorescence, immature embryo, shoot tip and shoot meristems of sorghum.

He worked on genetic transformation of sorghum using Bt gene constructs, assay of transformants for studying integration, expression and inheritance of transgenes.
He is an expert in designing PCR primers and oligos for custom microarray to study global gene expression in Vitis spps, Xylella fastidiosa, Bacillus anthracis and Phanerochaete chrysosporium. Experience in handling, printing, hybridization and scanning stages of microarrays.
He has extensive knowledge in using microarray data analysis software’s such as MEV, SAM and gene spring 4.0 and data interpretation. Experience in data integration with Gene ontologies and use of software’s like GenMAPP2 ermineJ and Pathway explorer.
He has good programming knowledge of PHP, PERL, HTML and MYSQL. Development of relational databases. comparative genomics, identifying specific DNA biomarkers for detection and differentiation of bacteria and fungi and other pathogens.
He has Knowledge of using a wide variety of computer software for phylogenomics and bioinformatics tools EST and genomic sequence analysis.