Expert Details

Expert in Biotechnology: All Areas of Business and Technology

Expert ID: 107913 Illinois, USA

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Expert has extensive hands-on experience with, and knowledge of, deoxyribonucleic acid (DNA) probe technology, having worked in this area for 10 years. He used nucleic acid probe technology to characterize mammalian viruses and oncogenes while performing research supported by the US National Cancer Institute. He also used DNA probe technology for his PhD research project and dissertation, which focused on the phylogeny and relationships of prosimian and simian primates as indicated by DNA-DNA hybridization.

Expert has extensive experience with industrial fermentation, biotechnology, genetic engineering, and related areas. Having consulted in these areas for 12 years, he has provided information on technical, business, economic, market, commercial, and strategic developments of microbial fermentation. Expert has focused on such products as antibiotics, amino acids, vitamins, organic acids, recombinant DNA therapeutics, industrial enzymes, foods and beverages, and other products produced by fermentation.

Expert has applied his background and hands-on experience in biotechnology, molecular genetics, molecular biology, and biochemistry to various industries worldwide. He is especially knowledgeable of the fermentation industry and has evaluated and designed industrial fermentation systems.He can advise clients on issues specific to antibiotic synthesis, genetic manipulation, organic acids, recombinant DNA, vitamins, and biotechnology assessment and modeling.

Having worked in the area of cancer research for 10 years (seven of which were connected to the US National Cancer Institute), Expert has hands-on experience with many aspects of cancer and the various cancer treatments. He has conducted laboratory research in chemical carcinogenesis. He has thoroughly investigated the effects of a single 20mg/kg intraperitoneal injection of 3-methylcholanthrene in adult male rats. He studied this extremely potent chemical carcinogen for liver 1) growth (including liver weight, total liver DNA and RNA, and total liver protein); 2) drug metabolizing enzyme activity, specifically 3-methyl-4-monomethylaminoazobenzene N-demethylase activity; 3) 15,000 x g supernatant RNA and protein content; and 4) free, membrane-bound, and total (free + membrane-bound) ribosomal RNA and protein content.

Expert has conducted extensive laboratory research on viral carcinogenesis and cancer genetics while working with the US National Cancer Institute. Particularly noteworthy is his expertise in obtaining, isolating, and characterizing viruses, viral genes, and oncogenes from primates and nonprimates. He has published several scientific papers on both chemical carcinogenesis and viral carcinogenesis. He also has an interest in tumor suppressor genes.

In addition to his many areas of expertise, Expert is also knowledgeable of the Cohn blood fractionation process which produces desirable fractionations and separations of blood using varying concentrations of ethanol at low temperatures. He has consulted with industry groups in the areas of blood fractionation and blood processing for the purpose of developing products for therapeutics, diagnostics, and research.

Recently, Expert applied DNA probe technology in the human disease diagnostics area for the Diagnostics Division at Abbott Laboratories, where he was responsible for product development and market entry of Abbott's Hepatitis B Viral (HBV) DNA diagnostic assay. He received the Abbott Laboratories' Presidential Award for his scientific work in developing this diagnostic product. He has several scientific publications on DNA probe technology to his credit. He has consulted for clients on various DNA issues. He can answer in-depth questions on DNA structure, synthesis, sequencing, and manipulation.


Expert has worked with enzymes for eight years. He has experience with industrial enzymes, human therapeutic enzymes, and restriction enzymes. He has purified enzymes used for human therapeutic purposes and for research purposes. Expert consults with clients on technical, business, economic, market, commercial, and strategic aspects of enzymes and the enzyme industry. He has worked with thermostable enzymes used in the fermentation industry and the pulp and paper industry. Expert organized and presided over two international conferences on industrial enzymes in Chicago. In addition, he has edited and published the proceedings of these conferences.

Expert has experience with chiral synthesis, chirality, and chiral drugs. He has consulted with pharmaceutical, biotechnology, agrochemical, and separation technology clients in these areas. He has focused on technologies for producing pure optical isomers out of racemates and on producing chiral intermediate compounds.

Expert is knowledgeable of nutraceuticals and how foods may be used to treat human disease. He has applied his background in biotechnology, molecular genetics, molecular biology, and biochemistry to the area of nutraceuticals across a wide range of industries.

Expert has experience with blood substitutes. He has consulted with clients on the technologies, applications, business aspects, and markets for blood substitutes.

Expert has worked with enzymes for eight years. He has experience with industrial enzymes, human therapeutic enzymes, and restriction enzymes. He has purified enzymes used for human therapeutic purposes and for research purposes. Expert consults with clients on technical, business, economic, market, commercial, and strategic aspects of enzymes and the enzyme industry. He has worked with thermostable enzymes used in the fermentation industry and the pulp and paper industry. Expert organized and presided over two recent international conferences on industrial enzymes in Chicago. In addition, he has edited and published the proceedings of these conferences.


Expert has extensive experience with process design, technology and market reviews, in-depth research projects, implementation assistance, etc.

Expert may consult nationally and internationally, and is also local to the following cities: Chicago, Illinois - Rockford, Illinois - Aurora, Illinois - Naperville, Illinois - Joliet, Illinois - Elgin, Illinois - South Bend, Indiana - Gary, Indiana - Milwaukee, Wisconsin - Madison, Wisconsin

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Education

Year Degree Subject Institution
Year: 1982 Degree: PhD Subject: Molecular Genetics/Molecular Biology Institution: The George Washington University
Year: 1973 Degree: MS Subject: Biochemistry/Molecular Biology Institution: Syracuse University
Year: 1969 Degree: BS Subject: Biology Institution: Syracuse University

Work History

Years Employer Title Department Responsibilities
Years: 1994 to Present Employer: Undisclosed Title: President and Consultant Department: Responsibilities:
Years: 1988 to 1994 Employer: Bernard Wolnak & Associates Title: Associate Consultant Department: Responsibilities:
Years: 1985 to 1988 Employer: Abbott Laboratories Title: Research Scientist Department: Diagnostics Division Responsibilities:
Years: 1983 to 1985 Employer: Smith Laboratories, Inc. Title: Manager of Biological Research Department: Responsibilities:
Years: 1982 to 1983 Employer: Georgetown University School of Med. Title: Postdoctoral Research Fellow Department: Responsibilities:
Years: 1980 to 1982 Employer: The George Washington University Title: Ph.D. Candidate Department: Responsibilities:
Years: 1973 to 1980 Employer: Meloy Laboratories, Inc. Title: Senior Research Biochemist Department: Responsibilities:
Years: 1972 to 1973 Employer: State University Of New York Upstate Medical Center Title: Research Scientist Department: Responsibilities:

International Experience

Years Country / Region Summary
Years: 1988 to Present Country / Region: Canada, Europe, Asia, Japan, and elsewhere. Summary: Over the years, Expert has consulted to clients outside the United States,including Canada, Europe, Asia, Japan, and elsewhere.

Career Accomplishments

Associations / Societies
Expert is a member of the American Association for the Advancement of Science, the Association of University Technology Managers, the American Chemical Society, and the Society for Industrial Microbiology.
Professional Appointments
Expert was appointed by the mayor of Manlius, New York to serve on the Conservation Advisory Council in upstate New York.
Awards / Recognition
Expert received the Abbott Laboratories Presidential Award for developing the Hepatitis B Viral DNA probe diagnostic assay.
Publications and Patents Summary
Expert has numerous scientific publications on a variety of subjects and several books on industrial enzymes.

Additional Experience

Training / Seminars
Expert teaches a seminar on biotechnology. This seminar is intended to help people whose work brings them into contact with the biotechnology and related industries, but who have limited or no training in the area. Topics covered include structure and function of cells and genes, DNA and how it is manipulated, genetic engineering, fermentation, proteins, recombinant DNA technology, gene splicing, and human gene therapy. Expert's seminar also provides an introduction to biotechnology as well as biotechnology used for diagnostics and therapeutics, medical, agricultural, food, and environmental biotechnology applications, and additional topics relating to biotechnology.
Vendor Selection
Expert has experience locating vendors of biotechnology services, biomedical services, diagnostic services, pharmaceutical services, cosmetics, fine chemicals, and agricultural (animal feed, veterinary) services.

Language Skills

Language Proficiency
Expert reads and writes Spanish and French. He has conducted scientific business in Belgium, Canada, China, Denmark, France, Greece, Israel, Italy, Japan, Mexico, Puerto Rico, Spain, Sweden, Switzerland, and the United Kingdom.

Fields of Expertise

DNA probe, DNA probe technology, DNA technology, genetic probe, DNA analysis, industrial biotechnology, industrial fermentation, applied biotechnology, cancer, cancer research, cancer treatment, cancerous tumor, carcinogenesis, blood fractionation, DNA manipulation, DNA assay, DNA synthesis, industrial enzyme, chiral synthesis, nutraceutical, artificial blood, enzyme engineering, enzyme purification, enzyme technology, animal feed industry, seed technology, yeast cloning vector, pharmaceutical technology, amino acid analysis, vaccine vector, cancer immunotherapy, cleaning enzyme, mutagenicity, transgenesis, DNA priming, colorectal cancer, restriction endonuclease, Generally Recognized As Safe, biohazardous material, plant genetic engineering, complementary DNA, alkaline phosphatase, glyoxalase, biotechnology assessment, amylase, cancer chemotherapy, enzyme stability, cellulase, food product nutrient enrichment, Southern blotting, enzyme activity assay, DNA probe, pulse field gel electrophoresis, cancer biology, extracellular enzyme, recombinant cell, biotechnology quality assurance, vitaminology, synthetic seed technology, plant tissue culture, clinical microbiology, food ingredient technology, antisense DNA, oligonucleotide, drug delivery, enzyme immobilization, coagulase, catalase, biohazard, food engineering, molecular chirality, breast cancer, bioremediation, Food and Drug Administration regulation, environmental microbiology, retrovirus, enzyme kinetics, DNA structure, biological pesticide, hepatitis B, polymerase chain reaction, food science, recombinant DNA technology, DNA, fungal yeast, genetic vector, transgenic technology, plant biotechnology, organic acid, monoclonal antibody, microbiology, medicinal plant, liposome, immunology, immunoassay, hybridoma, genetics, genetic probe technology, genetic manipulation, gene fusion, gel electrophoresis, food regulation, fluorescence, esterase, enzyme wastewater treatment, enzyme production, dehydrogenase, chemiluminescence, carcinogenicity, carcinogen, biotechnology modeling, biomedical sensor, bacteriology, aquaculture, applied microbiology, antibiotic

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